The physical chemistry of the enigmatic phospholipid diacylglycerol pyrophosphate

Liza Strawn, Amy Babb, Christa Testerink, Edgar Eduard Kooijman

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Phosphatidic acid (PA) is a lipid second messenger that is formed transiently in plants in response to different stress conditions, and plays a role in recruiting protein targets, ultimately enabling an adequate response. Intriguingly, this increase in PA concentration in plants is generally followed by an increase in the phospholipid diacylglycerolpyrophosphate (DGPP), via turnover of PA. Although DGPP has been shown to induce stress-related responses in plants, it is unclear to date what its molecular function is and how it exerts its effect. Here, we describe the physicochemical properties, i.e., effective molecular shape and charge, of DGPP. We find that unlike PA, which imparts a negative curvature stress to a (phospho)lipid bilayer, DGPP stabilizes the bilayer phase of phosphatidylethanolamine (PE), similar to the effect of phosphatidylcholine (PC). DGPP thus has zero curvature. The pKa2 of the phosphomonoester of DGPP is 7.44 ± 0.02 in a PC bilayer, compared to a pKa2 of 7.9 for PA. Replacement of half of the PC with PE decreases the pKa2 of DGPP to 6.71 ± 0.02, similar to the behavior previously described for PA and summarized in the electrostatic-hydrogen bond switch model. Implications for the potential function of DGPP in biomembranes are discussed.

Original languageEnglish
Article number40
JournalFrontiers in Plant Science
Volume3
DOIs
Publication statusPublished - 20 Mar 2012
Externally publishedYes

Keywords

  • Dgpp
  • Diacylglycerol pyrophosphate
  • Effective lipid shape
  • Electrostatic hydrogen bond switch
  • Ionization
  • Lipid second messenger
  • Phosphatidic acid
  • Phospholipid signaling

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