The phenotypic expression of QTLs for partial resistance to barley leaf rust during plant development

Lijuan Wang, Yajun Wang, Zhen Wang, T.C. Marcel, R.E. Niks, Xiaoquan Qi

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Partial resistance is generally considered to be a durable form of resistance. In barley, Rphq2, Rphq3 and Rphq4 have been identified as consistent quantitative trait loci (QTLs) for partial resistance to the barley leaf rust pathogen Puccinia hordei. These QTLs have been incorporated separately into the susceptible L94 and the partially resistant Vada barley genetic backgrounds to obtain two sets of near isogenic lines (NILs). Previous studies have shown that these QTLs are not effective at conferring disease resistance in all stages of plant development. In the present study, the two sets of QTL–NILs and the two recurrent parents, L94 and Vada, were evaluated for resistance to P. hordei isolate 1.2.1 simultaneously under greenhouse conditions from the first leaf to the flag leaf stage. Effect of the QTLs on resistance was measured by development rate of the pathogen, expressed as latency period (LP). The data show that Rphq2 prolongs LP at the seedling stage (the first and second leaf stages) but has almost no effect on disease resistance in adult plants. Rphq4 showed no effect on LP until the third leaf stage, whereas Rphq3 is consistently effective at prolonging LP from the first leaf to the flag leaf. The changes in the effectiveness of Rphq2 and Rphq4 happen at the barley tillering stage (the third to fourth leaf stages). These results indicate that multiple disease evaluations of a single plant by repeated inoculations of the fourth leaf to the flag leaf should be conducted to precisely estimate the effect of Rphq4. The present study confirms and describes in detail the plant development-dependent effectiveness of partial resistance genes and, consequently, will enable a more precise evaluation of partial resistance regulation during barley development
Original languageEnglish
Pages (from-to)857-864
JournalTheoretical and Applied Genetics
Volume121
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Plant Development
Quantitative Trait Loci
leaf rust
Hordeum
plant development
quantitative trait loci
barley
Disease Resistance
leaves
Puccinia hordei
Seedlings
disease resistance
pathogens
isogenic lines
tillering
mature plants
genetic background
Genes
greenhouses
seedlings

Keywords

  • age-related resistance
  • near-isogenic lines
  • puccinia-hordei
  • pseudomonas-syringae
  • disease-resistance
  • pyrenophora-teres
  • mosaic-virus
  • arabidopsis
  • gene
  • responses

Cite this

Wang, Lijuan ; Wang, Yajun ; Wang, Zhen ; Marcel, T.C. ; Niks, R.E. ; Qi, Xiaoquan. / The phenotypic expression of QTLs for partial resistance to barley leaf rust during plant development. In: Theoretical and Applied Genetics. 2010 ; Vol. 121, No. 5. pp. 857-864.
@article{8e09f07f89db42628865d7c37c80ec29,
title = "The phenotypic expression of QTLs for partial resistance to barley leaf rust during plant development",
abstract = "Partial resistance is generally considered to be a durable form of resistance. In barley, Rphq2, Rphq3 and Rphq4 have been identified as consistent quantitative trait loci (QTLs) for partial resistance to the barley leaf rust pathogen Puccinia hordei. These QTLs have been incorporated separately into the susceptible L94 and the partially resistant Vada barley genetic backgrounds to obtain two sets of near isogenic lines (NILs). Previous studies have shown that these QTLs are not effective at conferring disease resistance in all stages of plant development. In the present study, the two sets of QTL–NILs and the two recurrent parents, L94 and Vada, were evaluated for resistance to P. hordei isolate 1.2.1 simultaneously under greenhouse conditions from the first leaf to the flag leaf stage. Effect of the QTLs on resistance was measured by development rate of the pathogen, expressed as latency period (LP). The data show that Rphq2 prolongs LP at the seedling stage (the first and second leaf stages) but has almost no effect on disease resistance in adult plants. Rphq4 showed no effect on LP until the third leaf stage, whereas Rphq3 is consistently effective at prolonging LP from the first leaf to the flag leaf. The changes in the effectiveness of Rphq2 and Rphq4 happen at the barley tillering stage (the third to fourth leaf stages). These results indicate that multiple disease evaluations of a single plant by repeated inoculations of the fourth leaf to the flag leaf should be conducted to precisely estimate the effect of Rphq4. The present study confirms and describes in detail the plant development-dependent effectiveness of partial resistance genes and, consequently, will enable a more precise evaluation of partial resistance regulation during barley development",
keywords = "age-related resistance, near-isogenic lines, puccinia-hordei, pseudomonas-syringae, disease-resistance, pyrenophora-teres, mosaic-virus, arabidopsis, gene, responses",
author = "Lijuan Wang and Yajun Wang and Zhen Wang and T.C. Marcel and R.E. Niks and Xiaoquan Qi",
year = "2010",
doi = "10.1007/s00122-010-1355-0",
language = "English",
volume = "121",
pages = "857--864",
journal = "Theoretical and Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "5",

}

The phenotypic expression of QTLs for partial resistance to barley leaf rust during plant development. / Wang, Lijuan; Wang, Yajun; Wang, Zhen; Marcel, T.C.; Niks, R.E.; Qi, Xiaoquan.

In: Theoretical and Applied Genetics, Vol. 121, No. 5, 2010, p. 857-864.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The phenotypic expression of QTLs for partial resistance to barley leaf rust during plant development

AU - Wang, Lijuan

AU - Wang, Yajun

AU - Wang, Zhen

AU - Marcel, T.C.

AU - Niks, R.E.

AU - Qi, Xiaoquan

PY - 2010

Y1 - 2010

N2 - Partial resistance is generally considered to be a durable form of resistance. In barley, Rphq2, Rphq3 and Rphq4 have been identified as consistent quantitative trait loci (QTLs) for partial resistance to the barley leaf rust pathogen Puccinia hordei. These QTLs have been incorporated separately into the susceptible L94 and the partially resistant Vada barley genetic backgrounds to obtain two sets of near isogenic lines (NILs). Previous studies have shown that these QTLs are not effective at conferring disease resistance in all stages of plant development. In the present study, the two sets of QTL–NILs and the two recurrent parents, L94 and Vada, were evaluated for resistance to P. hordei isolate 1.2.1 simultaneously under greenhouse conditions from the first leaf to the flag leaf stage. Effect of the QTLs on resistance was measured by development rate of the pathogen, expressed as latency period (LP). The data show that Rphq2 prolongs LP at the seedling stage (the first and second leaf stages) but has almost no effect on disease resistance in adult plants. Rphq4 showed no effect on LP until the third leaf stage, whereas Rphq3 is consistently effective at prolonging LP from the first leaf to the flag leaf. The changes in the effectiveness of Rphq2 and Rphq4 happen at the barley tillering stage (the third to fourth leaf stages). These results indicate that multiple disease evaluations of a single plant by repeated inoculations of the fourth leaf to the flag leaf should be conducted to precisely estimate the effect of Rphq4. The present study confirms and describes in detail the plant development-dependent effectiveness of partial resistance genes and, consequently, will enable a more precise evaluation of partial resistance regulation during barley development

AB - Partial resistance is generally considered to be a durable form of resistance. In barley, Rphq2, Rphq3 and Rphq4 have been identified as consistent quantitative trait loci (QTLs) for partial resistance to the barley leaf rust pathogen Puccinia hordei. These QTLs have been incorporated separately into the susceptible L94 and the partially resistant Vada barley genetic backgrounds to obtain two sets of near isogenic lines (NILs). Previous studies have shown that these QTLs are not effective at conferring disease resistance in all stages of plant development. In the present study, the two sets of QTL–NILs and the two recurrent parents, L94 and Vada, were evaluated for resistance to P. hordei isolate 1.2.1 simultaneously under greenhouse conditions from the first leaf to the flag leaf stage. Effect of the QTLs on resistance was measured by development rate of the pathogen, expressed as latency period (LP). The data show that Rphq2 prolongs LP at the seedling stage (the first and second leaf stages) but has almost no effect on disease resistance in adult plants. Rphq4 showed no effect on LP until the third leaf stage, whereas Rphq3 is consistently effective at prolonging LP from the first leaf to the flag leaf. The changes in the effectiveness of Rphq2 and Rphq4 happen at the barley tillering stage (the third to fourth leaf stages). These results indicate that multiple disease evaluations of a single plant by repeated inoculations of the fourth leaf to the flag leaf should be conducted to precisely estimate the effect of Rphq4. The present study confirms and describes in detail the plant development-dependent effectiveness of partial resistance genes and, consequently, will enable a more precise evaluation of partial resistance regulation during barley development

KW - age-related resistance

KW - near-isogenic lines

KW - puccinia-hordei

KW - pseudomonas-syringae

KW - disease-resistance

KW - pyrenophora-teres

KW - mosaic-virus

KW - arabidopsis

KW - gene

KW - responses

U2 - 10.1007/s00122-010-1355-0

DO - 10.1007/s00122-010-1355-0

M3 - Article

VL - 121

SP - 857

EP - 864

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

IS - 5

ER -