The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans

C. Zhang, L. Liu, X. Wang, J.H. Vossen, G. Li, T. Li, Z. Zheng, J. Gao, Y. Guo, R.G.F. Visser, J. Junming Li, Y. Bai, Yongchen Du

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56 Citations (Scopus)

Abstract

Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).
Original languageEnglish
Pages (from-to)1353-1364
JournalTheoretical and Applied Genetics
Volume127
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • late blight resistance
  • potato late blight
  • powdery mildew resistance
  • disease resistance
  • r-gene
  • cultivated potato
  • avirulence genes
  • clonal lineage
  • tomato hybrid
  • evolution

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