The occurrence of internal (1,5)-linked arabinofuranose and arabinopyranose residues in arabinogalactan side chains from soybean pectic substances

M.M.H. Huisman, L.P. Brüll, J.E. Thomas-Oates, J. Haverkamp, H.A. Schols, A.G.J. Voragen

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Abstract

CDTA-extractable soybean pectic substances were subjected to enzymatic digestion with arabinogalactan degrading enzymes yielding a resistant polymeric pectic backbone and arabino-, galacto-, and arabinogalacto-oligomers. The complex digest was fractionated using size-exclusion chromatography. Monosaccharide composition analysis, HPAEC fractionation and MALDI-TOF MS analysis of the resulting fractions showed that each contained a mixture of oligosaccharides of essentially the same degree of polymerisation, composed of only arabinose and galactose. MALDI-TOF MS analysis was used for molecular mass screening of oligosaccharides in underivatised HPAEC fractions. The monosaccharide sequence and the branching pattern of oligosaccharides (degree of polymerisation from 4 to 8) were determined using linkage analysis and ES-CID tandem MS analysis of the per-O-methylated oligosaccharides in each of the HPAEC fractions. These analyses indicated the presence of common linear (1¿4)-linked galacto-oligosaccharides, and both linear and branched arabino-oligosaccharides. In addition, the results unambiguously showed the presence of oligosaccharides containing (1¿4)-linked galactose residues bearing an arabinopyranose residue as the non-reducing terminal residue, and a mixture of linear oligosaccharides constructed of (1¿4)-linked galactose residues interspersed with an internal (1¿5)-linked arabinofuranose residue. The consequences of these two new structural features of pectic arabinogalactan side chains are discussed
Original languageEnglish
Pages (from-to)103-114
JournalCarbohydrate Research : an international journal
Volume330
DOIs
Publication statusPublished - 2001

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