The nematode effector Mj-NEROSs interacts with Rieske's iron–sulfur protein influencing plastid ROS production to suppress plant immunity

Boris Stojilković, Hui Xiang, Yujin Chen, Muhammad Iqbal Maulana, Lander Bauters, Hans Van de Put, Kathy Steppe, Jinling Liao, Janice de Almeida Engler, Godelieve Gheysen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Root-knot nematodes (RKN; Meloidogyne species) are plant pathogens that introduce several effectors in their hosts to facilitate infection. The actual targets and functioning mechanism of these effectors largely remain unexplored. This study illuminates the role and interplay of the Meloidogyne javanica nematode effector ROS suppressor (Mj-NEROSs) within the host plant environment. Mj-NEROSs suppresses INF1-induced cell death as well as flg22-induced callose deposition and reactive oxygen species (ROS) production. A transcriptome analysis highlighted the down regulation of ROS-related genes upon Mj-NEROSs expression. NEROSs interacts with the plant Rieske's iron–sulfur protein (ISP) as shown by yeast-two-hybrid and bimolecular fluorescence complementation. Secreted from the sub ventral pharyngeal glands into giant cells, Mj-NEROSs localizes in the plastids where it interacts with ISP, subsequently altering electron transport rates and ROS production. Moreover, our results demonstrate that isp Arabidopsis thaliana mutants exhibit increased susceptibility to M. javanica, indicating ISP importance for plant immunity. The interaction of a nematode effect or with a plastid protein highlights the possible role of root plastids in plant defense, prompting many questions on the details of this process.
Original languageEnglish
Article number19781
Pages (from-to)2787-2802
Number of pages16
JournalNew Phytologist
Volume242
Issue number6
Early online date1 May 2024
DOIs
Publication statusPublished - 2024

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