The nature of the bond between peptide and carrier molecule determines immunogenicity of the construct

N.L.C.M. Beekman, W.M.M. Schaaper, J.P.M. Langeveld, R.S. Boshuizen, R.H. Meloen

    Research output: Contribution to journalArticleAcademicpeer-review

    10 Citations (Scopus)

    Abstract

    The influence of the nature of the bond between a peptide and a (lipidic) carrier molecule on the immunogenicity of that construct was investigated. As types of bonds a thioester-, a disulfide-, an amide- and a thioether bond were investigated. As carrier molecules a peptide, an N-palmitoylated peptide or a C16-hydrocarbon chain were used. The biostability of the bond between peptide and carrier molecule is thioether > amide > disulfide thioester. However, the immunogenic potency of the constructs used was found to be thioester > disulfide > amide > thioether. In conclusion, a construct with a bond between peptide and (lipidic) carrier molecule that is more susceptible to biological degradation is more immunogenic when used in a peptide-based vaccine than a bond that is less susceptible to biological degradation.
    Original languageEnglish
    Pages (from-to)237-245
    JournalJournal of peptide research
    Volume58
    Issue number3
    DOIs
    Publication statusPublished - 2001

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