The movement protein of Cowpea mosaic virus binds GTP and single stranded nucleic acid in vitro

C.M. Carvalho; J. Pouwels; J.W.M. van Lent; T. Bisseling; R.W. Goldbach; J. Wellink

doi:10.1128/JVI.78.3.1591-1594.2004

The movement protein of Cowpea mosaic virus binds GTP and single stranded nucleic acid in vitro

C.M. Carvalho, J. Pouwels, J.W.M. van Lent, T. Bisseling, R.W. Goldbach, J. Wellink

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a nonsequence-specific manner, and the GTP- and RNA-binding sites do not overlap.

Original language	English
Pages (from-to)	1591-1594
Number of pages	4
Journal	Journal of Virology
Volume	78
Issue number	3
DOIs	https://doi.org/10.1128/JVI.78.3.1591-1594.2004
Publication status	Published - 2004

Keywords

cell-to-cell
escherichia-coli
rna
replication
microtubule
sequence
plants
transport

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10.1128/JVI.78.3.1591-1594.2004

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title = "The movement protein of Cowpea mosaic virus binds GTP and single stranded nucleic acid in vitro",

abstract = "The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a nonsequence-specific manner, and the GTP- and RNA-binding sites do not overlap.",

keywords = "cell-to-cell, escherichia-coli, rna, replication, microtubule, sequence, plants, transport",

author = "C.M. Carvalho and J. Pouwels and {van Lent}, J.W.M. and T. Bisseling and R.W. Goldbach and J. Wellink",

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doi = "10.1128/JVI.78.3.1591-1594.2004",

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T1 - The movement protein of Cowpea mosaic virus binds GTP and single stranded nucleic acid in vitro

AU - Carvalho, C.M.

AU - Pouwels, J.

AU - van Lent, J.W.M.

AU - Bisseling, T.

AU - Goldbach, R.W.

AU - Wellink, J.

PY - 2004

Y1 - 2004

N2 - The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a nonsequence-specific manner, and the GTP- and RNA-binding sites do not overlap.

AB - The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a nonsequence-specific manner, and the GTP- and RNA-binding sites do not overlap.

KW - cell-to-cell

KW - escherichia-coli

KW - rna

KW - replication

KW - microtubule

KW - sequence

KW - plants

KW - transport

U2 - 10.1128/JVI.78.3.1591-1594.2004

DO - 10.1128/JVI.78.3.1591-1594.2004

M3 - Article

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VL - 78

SP - 1591

EP - 1594

JO - Journal of Virology

JF - Journal of Virology

IS - 3

ER -

The movement protein of Cowpea mosaic virus binds GTP and single stranded nucleic acid in vitro

Abstract

Keywords

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