The Medicago truncatula nodule identity gene MtNOOT1 is required for coordinated apical-basal development of the root

Defeng Shen, Olga Kulikova, Kerstin Guhl, Henk Franssen, Wouter Kohlen, Ton Bisseling, René Geurts*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Legumes can utilize atmospheric nitrogen by hosting nitrogen-fixing bacteria in special lateral root organs, called nodules. Legume nodules have a unique ontology, despite similarities in the gene networks controlling nodule and lateral root development. It has been shown that Medicago truncatula NODULE ROOT1 (MtNOOT1) is required for the maintenance of nodule identity, preventing the conversion to lateral root development. MtNOOT1 and its orthologs in other plant species -collectively called the NOOT-BOP-COCH-LIKE (NBCL) family- specify boundary formation in various aerial organs. However, MtNOOT1 is not only expressed in nodules and aerial organs, but also in developing roots, where its function remains elusive. Results: We show that Mtnoot1 mutant seedlings display accelerated root elongation due to an enlarged root apical meristem. Also, Mtnoot1 mutant roots are thinner than wild-type and are delayed in xylem cell differentiation. We provide molecular evidence that the affected spatial development of Mtnoot1 mutant roots correlates with delayed induction of genes involved in xylem cell differentiation. This coincides with a basipetal shift of the root zone that is susceptible to rhizobium-secreted symbiotic signal molecules. Conclusions: Our data show that MtNOOT1 regulates the size of the root apical meristem and vascular differentiation. Our data demonstrate that MtNOOT1 not only functions as a homeotic gene in nodule development but also coordinates the spatial development of the root.

Original languageEnglish
Article number571
JournalBMC Plant Biology
Volume19
Issue number1
DOIs
Publication statusPublished - 19 Dec 2019

Fingerprint

Medicago truncatula
genes
root meristems
apical meristems
cell differentiation
mutants
xylem
legumes
nitrogen-fixing bacteria
homeotic genes
gene induction
blood vessels
Rhizobium
root growth
rhizosphere
seedlings
nitrogen

Keywords

  • Medicago truncatula
  • NBCL
  • NIN
  • NOOT-BOP-COCHLEATA-LIKE
  • NOOT1
  • Rhizobium susceptible zone
  • Xylem cell differentiation

Cite this

@article{4e757634d1274924b12509710bcd5cc7,
title = "The Medicago truncatula nodule identity gene MtNOOT1 is required for coordinated apical-basal development of the root",
abstract = "Background: Legumes can utilize atmospheric nitrogen by hosting nitrogen-fixing bacteria in special lateral root organs, called nodules. Legume nodules have a unique ontology, despite similarities in the gene networks controlling nodule and lateral root development. It has been shown that Medicago truncatula NODULE ROOT1 (MtNOOT1) is required for the maintenance of nodule identity, preventing the conversion to lateral root development. MtNOOT1 and its orthologs in other plant species -collectively called the NOOT-BOP-COCH-LIKE (NBCL) family- specify boundary formation in various aerial organs. However, MtNOOT1 is not only expressed in nodules and aerial organs, but also in developing roots, where its function remains elusive. Results: We show that Mtnoot1 mutant seedlings display accelerated root elongation due to an enlarged root apical meristem. Also, Mtnoot1 mutant roots are thinner than wild-type and are delayed in xylem cell differentiation. We provide molecular evidence that the affected spatial development of Mtnoot1 mutant roots correlates with delayed induction of genes involved in xylem cell differentiation. This coincides with a basipetal shift of the root zone that is susceptible to rhizobium-secreted symbiotic signal molecules. Conclusions: Our data show that MtNOOT1 regulates the size of the root apical meristem and vascular differentiation. Our data demonstrate that MtNOOT1 not only functions as a homeotic gene in nodule development but also coordinates the spatial development of the root.",
keywords = "Medicago truncatula, NBCL, NIN, NOOT-BOP-COCHLEATA-LIKE, NOOT1, Rhizobium susceptible zone, Xylem cell differentiation",
author = "Defeng Shen and Olga Kulikova and Kerstin Guhl and Henk Franssen and Wouter Kohlen and Ton Bisseling and Ren{\'e} Geurts",
year = "2019",
month = "12",
day = "19",
doi = "10.1186/s12870-019-2194-z",
language = "English",
volume = "19",
journal = "BMC Plant Biology",
issn = "1471-2229",
publisher = "Springer Verlag",
number = "1",

}

The Medicago truncatula nodule identity gene MtNOOT1 is required for coordinated apical-basal development of the root. / Shen, Defeng; Kulikova, Olga; Guhl, Kerstin; Franssen, Henk; Kohlen, Wouter; Bisseling, Ton; Geurts, René.

In: BMC Plant Biology, Vol. 19, No. 1, 571, 19.12.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The Medicago truncatula nodule identity gene MtNOOT1 is required for coordinated apical-basal development of the root

AU - Shen, Defeng

AU - Kulikova, Olga

AU - Guhl, Kerstin

AU - Franssen, Henk

AU - Kohlen, Wouter

AU - Bisseling, Ton

AU - Geurts, René

PY - 2019/12/19

Y1 - 2019/12/19

N2 - Background: Legumes can utilize atmospheric nitrogen by hosting nitrogen-fixing bacteria in special lateral root organs, called nodules. Legume nodules have a unique ontology, despite similarities in the gene networks controlling nodule and lateral root development. It has been shown that Medicago truncatula NODULE ROOT1 (MtNOOT1) is required for the maintenance of nodule identity, preventing the conversion to lateral root development. MtNOOT1 and its orthologs in other plant species -collectively called the NOOT-BOP-COCH-LIKE (NBCL) family- specify boundary formation in various aerial organs. However, MtNOOT1 is not only expressed in nodules and aerial organs, but also in developing roots, where its function remains elusive. Results: We show that Mtnoot1 mutant seedlings display accelerated root elongation due to an enlarged root apical meristem. Also, Mtnoot1 mutant roots are thinner than wild-type and are delayed in xylem cell differentiation. We provide molecular evidence that the affected spatial development of Mtnoot1 mutant roots correlates with delayed induction of genes involved in xylem cell differentiation. This coincides with a basipetal shift of the root zone that is susceptible to rhizobium-secreted symbiotic signal molecules. Conclusions: Our data show that MtNOOT1 regulates the size of the root apical meristem and vascular differentiation. Our data demonstrate that MtNOOT1 not only functions as a homeotic gene in nodule development but also coordinates the spatial development of the root.

AB - Background: Legumes can utilize atmospheric nitrogen by hosting nitrogen-fixing bacteria in special lateral root organs, called nodules. Legume nodules have a unique ontology, despite similarities in the gene networks controlling nodule and lateral root development. It has been shown that Medicago truncatula NODULE ROOT1 (MtNOOT1) is required for the maintenance of nodule identity, preventing the conversion to lateral root development. MtNOOT1 and its orthologs in other plant species -collectively called the NOOT-BOP-COCH-LIKE (NBCL) family- specify boundary formation in various aerial organs. However, MtNOOT1 is not only expressed in nodules and aerial organs, but also in developing roots, where its function remains elusive. Results: We show that Mtnoot1 mutant seedlings display accelerated root elongation due to an enlarged root apical meristem. Also, Mtnoot1 mutant roots are thinner than wild-type and are delayed in xylem cell differentiation. We provide molecular evidence that the affected spatial development of Mtnoot1 mutant roots correlates with delayed induction of genes involved in xylem cell differentiation. This coincides with a basipetal shift of the root zone that is susceptible to rhizobium-secreted symbiotic signal molecules. Conclusions: Our data show that MtNOOT1 regulates the size of the root apical meristem and vascular differentiation. Our data demonstrate that MtNOOT1 not only functions as a homeotic gene in nodule development but also coordinates the spatial development of the root.

KW - Medicago truncatula

KW - NBCL

KW - NIN

KW - NOOT-BOP-COCHLEATA-LIKE

KW - NOOT1

KW - Rhizobium susceptible zone

KW - Xylem cell differentiation

U2 - 10.1186/s12870-019-2194-z

DO - 10.1186/s12870-019-2194-z

M3 - Article

VL - 19

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

IS - 1

M1 - 571

ER -