The MADS domain protein DIANA together with AGAMOUS-LIKE80 to specify the central cell in Arabidopsis ovules

M. Bemer, M. Wolters-Arts, U. Grossniklaus, G.C. Angenent

    Research output: Contribution to journalArticleAcademicpeer-review

    81 Citations (Scopus)

    Abstract

    MADS box genes in plants consist of MIKC-type and type I genes. While MIKC-type genes have been studied extensively, the functions of type I genes are still poorly understood. Evidence suggests that type I MADS box genes are involved in embryo sac and seed development. We investigated two independent T-DNA insertion alleles of the Arabidopsis thaliana type I MADS box gene AGAMOUS-LIKE61 (AGL61) and showed that in agl61 mutant ovules, the polar nuclei do not fuse and central cell morphology is aberrant. Furthermore, the central cell begins to degenerate before fertilization takes place. Although pollen tubes are attracted and perceived by the mutant ovules, neither endosperm development nor zygote formation occurs. AGL61 is expressed in the central cell during the final stages of embryo sac development. An AGL61:green fluorescent protein¿-glucoronidase fusion protein localizes exclusively to the polar nuclei and the secondary nucleus of the central cell. Yeast two-hybrid analysis showed that AGL61 can form a heterodimer with AGL80 and that the nuclear localization of AGL61 is lost in the agl80 mutant. Thus, AGL61 and AGL80 appear to function together to differentiate the central cell in Arabidopsis. We renamed AGL61 DIANA, after the virginal Roman goddess of the hunt.
    Original languageEnglish
    Pages (from-to)2088-2101
    JournalThe Plant Cell
    Volume20
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    MADS Domain Proteins
    Ovule
    Arabidopsis
    ovules
    Genes
    genes
    proteins
    embryo sac
    cells
    mutants
    Pollen Tube
    Plant Genes
    Endosperm
    Zygote
    two hybrid system techniques
    Green Fluorescent Proteins
    Cell Nucleus
    Fertilization
    Embryonic Development
    zygote

    Keywords

    • female gametophyte development
    • polycomb group gene
    • seed development
    • box gene
    • endosperm development
    • wild-type
    • thaliana
    • identification
    • fertilization
    • expression

    Cite this

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    abstract = "MADS box genes in plants consist of MIKC-type and type I genes. While MIKC-type genes have been studied extensively, the functions of type I genes are still poorly understood. Evidence suggests that type I MADS box genes are involved in embryo sac and seed development. We investigated two independent T-DNA insertion alleles of the Arabidopsis thaliana type I MADS box gene AGAMOUS-LIKE61 (AGL61) and showed that in agl61 mutant ovules, the polar nuclei do not fuse and central cell morphology is aberrant. Furthermore, the central cell begins to degenerate before fertilization takes place. Although pollen tubes are attracted and perceived by the mutant ovules, neither endosperm development nor zygote formation occurs. AGL61 is expressed in the central cell during the final stages of embryo sac development. An AGL61:green fluorescent protein¿-glucoronidase fusion protein localizes exclusively to the polar nuclei and the secondary nucleus of the central cell. Yeast two-hybrid analysis showed that AGL61 can form a heterodimer with AGL80 and that the nuclear localization of AGL61 is lost in the agl80 mutant. Thus, AGL61 and AGL80 appear to function together to differentiate the central cell in Arabidopsis. We renamed AGL61 DIANA, after the virginal Roman goddess of the hunt.",
    keywords = "female gametophyte development, polycomb group gene, seed development, box gene, endosperm development, wild-type, thaliana, identification, fertilization, expression",
    author = "M. Bemer and M. Wolters-Arts and U. Grossniklaus and G.C. Angenent",
    year = "2008",
    doi = "10.1105/tpc.108.058958",
    language = "English",
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    issn = "1040-4651",
    publisher = "American Society of Plant Biologists",

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    The MADS domain protein DIANA together with AGAMOUS-LIKE80 to specify the central cell in Arabidopsis ovules. / Bemer, M.; Wolters-Arts, M.; Grossniklaus, U.; Angenent, G.C.

    In: The Plant Cell, Vol. 20, 2008, p. 2088-2101.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - The MADS domain protein DIANA together with AGAMOUS-LIKE80 to specify the central cell in Arabidopsis ovules

    AU - Bemer, M.

    AU - Wolters-Arts, M.

    AU - Grossniklaus, U.

    AU - Angenent, G.C.

    PY - 2008

    Y1 - 2008

    N2 - MADS box genes in plants consist of MIKC-type and type I genes. While MIKC-type genes have been studied extensively, the functions of type I genes are still poorly understood. Evidence suggests that type I MADS box genes are involved in embryo sac and seed development. We investigated two independent T-DNA insertion alleles of the Arabidopsis thaliana type I MADS box gene AGAMOUS-LIKE61 (AGL61) and showed that in agl61 mutant ovules, the polar nuclei do not fuse and central cell morphology is aberrant. Furthermore, the central cell begins to degenerate before fertilization takes place. Although pollen tubes are attracted and perceived by the mutant ovules, neither endosperm development nor zygote formation occurs. AGL61 is expressed in the central cell during the final stages of embryo sac development. An AGL61:green fluorescent protein¿-glucoronidase fusion protein localizes exclusively to the polar nuclei and the secondary nucleus of the central cell. Yeast two-hybrid analysis showed that AGL61 can form a heterodimer with AGL80 and that the nuclear localization of AGL61 is lost in the agl80 mutant. Thus, AGL61 and AGL80 appear to function together to differentiate the central cell in Arabidopsis. We renamed AGL61 DIANA, after the virginal Roman goddess of the hunt.

    AB - MADS box genes in plants consist of MIKC-type and type I genes. While MIKC-type genes have been studied extensively, the functions of type I genes are still poorly understood. Evidence suggests that type I MADS box genes are involved in embryo sac and seed development. We investigated two independent T-DNA insertion alleles of the Arabidopsis thaliana type I MADS box gene AGAMOUS-LIKE61 (AGL61) and showed that in agl61 mutant ovules, the polar nuclei do not fuse and central cell morphology is aberrant. Furthermore, the central cell begins to degenerate before fertilization takes place. Although pollen tubes are attracted and perceived by the mutant ovules, neither endosperm development nor zygote formation occurs. AGL61 is expressed in the central cell during the final stages of embryo sac development. An AGL61:green fluorescent protein¿-glucoronidase fusion protein localizes exclusively to the polar nuclei and the secondary nucleus of the central cell. Yeast two-hybrid analysis showed that AGL61 can form a heterodimer with AGL80 and that the nuclear localization of AGL61 is lost in the agl80 mutant. Thus, AGL61 and AGL80 appear to function together to differentiate the central cell in Arabidopsis. We renamed AGL61 DIANA, after the virginal Roman goddess of the hunt.

    KW - female gametophyte development

    KW - polycomb group gene

    KW - seed development

    KW - box gene

    KW - endosperm development

    KW - wild-type

    KW - thaliana

    KW - identification

    KW - fertilization

    KW - expression

    U2 - 10.1105/tpc.108.058958

    DO - 10.1105/tpc.108.058958

    M3 - Article

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    SP - 2088

    EP - 2101

    JO - The Plant Cell

    JF - The Plant Cell

    SN - 1040-4651

    ER -