TY - JOUR
T1 - The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation
AU - Beukema, M.
AU - Jermendi, null
AU - Oerlemans, M.M.P.
AU - Logtenberg, M.J.
AU - Akkerman, R.
AU - An, R.
AU - van den Berg, M.A.
AU - Zoetendal, E.G.
AU - Koster, T.
AU - Kong, C.
AU - Faas, M.M.
AU - Schols, H.A.
AU - de Vos, P.
PY - 2022/6/15
Y1 - 2022/6/15
N2 - Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown. Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not. After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased. These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins. T-cell-modulating effects were not short-chain-fatty acid-dependent, but rather through an increase in Aryl-hydrocarbon-receptor-activating components. Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.
AB - Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown. Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not. After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt+ regulatory T-cell frequencies increased. These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins. T-cell-modulating effects were not short-chain-fatty acid-dependent, but rather through an increase in Aryl-hydrocarbon-receptor-activating components. Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose.
KW - Aryl-hydrocarbon receptor
KW - Degree of blockiness
KW - Degree of methyl-esterification
KW - Microbiota
KW - Pectin
KW - Short-chain fatty acids
KW - T cell
U2 - 10.1016/j.carbpol.2022.119280
DO - 10.1016/j.carbpol.2022.119280
M3 - Article
AN - SCOPUS:85125012559
SN - 0144-8617
VL - 286
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 119280
ER -