Abstract
Phytophthora infestans secretes many RXLR effectors, one of which is IPI-O. The RXLR
motif of IPI-O is in partial overlap with an RGD cell adhesion motif (RSLRGD), which has
been shown to bind LecRK-I.9, a lectin receptor kinase of Arabidopsis. LecRK-I.9 was
shown to mediate the adhesions between the cell wall (CW) and plasma membrane (PM),
and it has been observed that IPI-O can disrupt these adhesions through its RGD motif.
Transgenic Arabidopsis lines expressing ipiO and Arabidopsis LecRK-I.9 knock-out lines
(lecrk-I.9) were analyzed in their response to pathogen infection, in particular to
Phytophthora. Both, the ipiO expressing lines and lecrk-I.9 are impaired in their resistance to
oomycete pathogens. To unravel the mechanisms underlying this phenomenon we analysed
callose deposition upon MAMP treatment and investigated the strength of cell wall-plasma
membrane adhesions by inducing plasmolysis. The obtained results indicate that LecRK-I.9
is not only important for the maintenance of the CW-PM continuum, but also in MAMPtriggered
immunity. Moreover, Arabidopsis lines with constitutive LecRK-I.9 expression
display an enhanced Phytophthora resistance. Our observations strongly suggest that LecRKI.
9 plays a crucial role in disease resistance and point toward involvement of the RXLR
effector IPI-O in the infection process
| Original language | English |
|---|---|
| Title of host publication | Book of Abstracts Oomycete Molecular Genetics Network Congress, Toulouse, France, 6-8 June 2010 |
| Pages | 34-34 |
| Publication status | Published - 2010 |
| Event | Oomycete Molecular Genetics Network Congress, Toulouse, France - Duration: 6 Jun 2010 → 8 Jun 2010 |
Conference/symposium
| Conference/symposium | Oomycete Molecular Genetics Network Congress, Toulouse, France |
|---|---|
| Period | 6/06/10 → 8/06/10 |