The lectin receptor LecRK-I.9 is a novel Phytophthora resistance component and a host target for the RXLR effector IPI-O.

K. Bouwmeester, M. de Sain, R. Weide, H. Canut, F. Govers

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

Phytophthora infestans secretes many RXLR effectors, one of which is IPI-O. The RXLR motif of IPI-O is in partial overlap with an RGD cell adhesion motif (RSLRGD), which has been shown to bind LecRK-I.9, a lectin receptor kinase of Arabidopsis. LecRK-I.9 was shown to mediate the adhesions between the cell wall (CW) and plasma membrane (PM), and it has been observed that IPI-O can disrupt these adhesions through its RGD motif. Transgenic Arabidopsis lines expressing ipiO and Arabidopsis LecRK-I.9 knock-out lines (lecrk-I.9) were analyzed in their response to pathogen infection, in particular to Phytophthora. Both, the ipiO expressing lines and lecrk-I.9 are impaired in their resistance to oomycete pathogens. To unravel the mechanisms underlying this phenomenon we analysed callose deposition upon MAMP treatment and investigated the strength of cell wall-plasma membrane adhesions by inducing plasmolysis. The obtained results indicate that LecRK-I.9 is not only important for the maintenance of the CW-PM continuum, but also in MAMPtriggered immunity. Moreover, Arabidopsis lines with constitutive LecRK-I.9 expression display an enhanced Phytophthora resistance. Our observations strongly suggest that LecRKI. 9 plays a crucial role in disease resistance and point toward involvement of the RXLR effector IPI-O in the infection process
Original languageEnglish
Title of host publicationBook of Abstracts Oomycete Molecular Genetics Network Congress, Toulouse, France, 6-8 June 2010
Pages34-34
Publication statusPublished - 2010
EventOomycete Molecular Genetics Network Congress, Toulouse, France -
Duration: 6 Jun 20108 Jun 2010

Conference

ConferenceOomycete Molecular Genetics Network Congress, Toulouse, France
Period6/06/108/06/10

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