The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica)

Stefano Pessina, Dario Angeli, Stefan Martens, Richard G.F. Visser, Yuling Bai, Francesco Salamini, Riccardo Velasco, Henk J. Schouten, Mickael Malnoy

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Varieties resistant to powdery mildew (PM; caused by Podosphaera leucotricha) are a major component of sustainable apple production. Resistance can be achieved by knocking-out susceptibility S-genes to be singled out among members of the MLO (Mildew Locus O) gene family. Candidates are MLO S-genes of phylogenetic clade V up-regulated upon PM inoculation, such as MdMLO11 and 19 (clade V) and MdMLO18 (clade VII). We report the knock-down through RNA interference of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant Atmlo2/6/12. The knock-down of MdMLO19 reduced PM disease severity by 75%, whereas the knock-down of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility compared with MdMLO19 alone. The test in A. thaliana excluded a role for MdMLO18 in PM susceptibility. Cell wall appositions (papillae) were present in both PM-resistant and PM-susceptible plants, but were larger in resistant lines. No obvious negative phenotype was observed in plants with mlo genes knocked down. Apparently, MdMLO19 plays the pivotal role in apple PM susceptibility and its knock-down induces a very significant level of resistance.

Original languageEnglish
Pages (from-to)2033-2044
JournalPlant Biotechnology Journal
Volume14
Issue number10
DOIs
Publication statusPublished - 2016

Fingerprint

Podosphaera leucotricha
Malus
Malus domestica
powdery mildew
apples
Arabidopsis
Genes
genes
Arabidopsis thaliana
loci
RNA Interference
RNA interference
Cell Wall
disease severity
cell walls
Phenotype
phenotype
mutants
phylogeny
testing

Keywords

  • Arabidopsis thaliana
  • Malus domestica
  • MLO
  • Powdery mildew
  • RNA interference

Cite this

Pessina, Stefano ; Angeli, Dario ; Martens, Stefan ; Visser, Richard G.F. ; Bai, Yuling ; Salamini, Francesco ; Velasco, Riccardo ; Schouten, Henk J. ; Malnoy, Mickael. / The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica). In: Plant Biotechnology Journal. 2016 ; Vol. 14, No. 10. pp. 2033-2044.
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title = "The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica)",
abstract = "Varieties resistant to powdery mildew (PM; caused by Podosphaera leucotricha) are a major component of sustainable apple production. Resistance can be achieved by knocking-out susceptibility S-genes to be singled out among members of the MLO (Mildew Locus O) gene family. Candidates are MLO S-genes of phylogenetic clade V up-regulated upon PM inoculation, such as MdMLO11 and 19 (clade V) and MdMLO18 (clade VII). We report the knock-down through RNA interference of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant Atmlo2/6/12. The knock-down of MdMLO19 reduced PM disease severity by 75{\%}, whereas the knock-down of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility compared with MdMLO19 alone. The test in A. thaliana excluded a role for MdMLO18 in PM susceptibility. Cell wall appositions (papillae) were present in both PM-resistant and PM-susceptible plants, but were larger in resistant lines. No obvious negative phenotype was observed in plants with mlo genes knocked down. Apparently, MdMLO19 plays the pivotal role in apple PM susceptibility and its knock-down induces a very significant level of resistance.",
keywords = "Arabidopsis thaliana, Malus domestica, MLO, Powdery mildew, RNA interference",
author = "Stefano Pessina and Dario Angeli and Stefan Martens and Visser, {Richard G.F.} and Yuling Bai and Francesco Salamini and Riccardo Velasco and Schouten, {Henk J.} and Mickael Malnoy",
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The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica). / Pessina, Stefano; Angeli, Dario; Martens, Stefan; Visser, Richard G.F.; Bai, Yuling; Salamini, Francesco; Velasco, Riccardo; Schouten, Henk J.; Malnoy, Mickael.

In: Plant Biotechnology Journal, Vol. 14, No. 10, 2016, p. 2033-2044.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The knock-down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica)

AU - Pessina, Stefano

AU - Angeli, Dario

AU - Martens, Stefan

AU - Visser, Richard G.F.

AU - Bai, Yuling

AU - Salamini, Francesco

AU - Velasco, Riccardo

AU - Schouten, Henk J.

AU - Malnoy, Mickael

PY - 2016

Y1 - 2016

N2 - Varieties resistant to powdery mildew (PM; caused by Podosphaera leucotricha) are a major component of sustainable apple production. Resistance can be achieved by knocking-out susceptibility S-genes to be singled out among members of the MLO (Mildew Locus O) gene family. Candidates are MLO S-genes of phylogenetic clade V up-regulated upon PM inoculation, such as MdMLO11 and 19 (clade V) and MdMLO18 (clade VII). We report the knock-down through RNA interference of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant Atmlo2/6/12. The knock-down of MdMLO19 reduced PM disease severity by 75%, whereas the knock-down of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility compared with MdMLO19 alone. The test in A. thaliana excluded a role for MdMLO18 in PM susceptibility. Cell wall appositions (papillae) were present in both PM-resistant and PM-susceptible plants, but were larger in resistant lines. No obvious negative phenotype was observed in plants with mlo genes knocked down. Apparently, MdMLO19 plays the pivotal role in apple PM susceptibility and its knock-down induces a very significant level of resistance.

AB - Varieties resistant to powdery mildew (PM; caused by Podosphaera leucotricha) are a major component of sustainable apple production. Resistance can be achieved by knocking-out susceptibility S-genes to be singled out among members of the MLO (Mildew Locus O) gene family. Candidates are MLO S-genes of phylogenetic clade V up-regulated upon PM inoculation, such as MdMLO11 and 19 (clade V) and MdMLO18 (clade VII). We report the knock-down through RNA interference of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant Atmlo2/6/12. The knock-down of MdMLO19 reduced PM disease severity by 75%, whereas the knock-down of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility compared with MdMLO19 alone. The test in A. thaliana excluded a role for MdMLO18 in PM susceptibility. Cell wall appositions (papillae) were present in both PM-resistant and PM-susceptible plants, but were larger in resistant lines. No obvious negative phenotype was observed in plants with mlo genes knocked down. Apparently, MdMLO19 plays the pivotal role in apple PM susceptibility and its knock-down induces a very significant level of resistance.

KW - Arabidopsis thaliana

KW - Malus domestica

KW - MLO

KW - Powdery mildew

KW - RNA interference

U2 - 10.1111/pbi.12562

DO - 10.1111/pbi.12562

M3 - Article

VL - 14

SP - 2033

EP - 2044

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

SN - 1467-7644

IS - 10

ER -