The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor

Philip D. Townsend; Christopher H. Dixon; Erik J. Slootweg; Octavina C.A. Sukarta; Ally W.H. Yang; Timothy R. Hughes; Gary J. Sharples; Lars-Olof Palsson; Frank L.W. Takken; Aska Goverse; Martin J. Cann

doi:10.1074/jbc.RA117.000485

The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor

Philip D. Townsend, Christopher H. Dixon, Erik J. Slootweg, Octavina C.A. Sukarta, Ally W.H. Yang, Timothy R. Hughes, Gary J. Sharples, Lars-Olof Palsson, Frank L.W. Takken, Aska Goverse, Martin J. Cann^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

47 Citations (Scopus)

Abstract

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato is further able to bind and distort double-stranded DNA. However, Rx1 host targets that support a role for Rx1 in transcriptional reprogramming at DNA are unknown. Here we report a functional interaction between Rx1 and NbGlk1, a Golden2- like transcription factor. Rx1 binds to NbGlk1 in vitro and in planta. NbGlk1 binds to known Golden2-like consensus DNA sequences. Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro. NbGlk1 activates cellular responses to potato virus X, whereas Rx1 associates with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX. This study provides new mechanistic insight into how an NLR can co-ordinate an immune signalling response at DNA following pathogen perception.

Original language	English
Pages (from-to)	3218-3233
Journal	Journal of Biological Chemistry
Volume	293
Issue number	9
Early online date	7 Dec 2017
DOIs	https://doi.org/10.1074/jbc.RA117.000485
Publication status	Published - 2 Mar 2018

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Townsend, P. D., Dixon, C. H., Slootweg, E. J., Sukarta, O. C. A., Yang, A. W. H., Hughes, T. R., Sharples, G. J., Palsson, L.-O., Takken, F. L. W., Goverse, A., & Cann, M. J. (2018). The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. Journal of Biological Chemistry, 293(9), 3218-3233. https://doi.org/10.1074/jbc.RA117.000485

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title = "The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor",

abstract = "Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato is further able to bind and distort double-stranded DNA. However, Rx1 host targets that support a role for Rx1 in transcriptional reprogramming at DNA are unknown. Here we report a functional interaction between Rx1 and NbGlk1, a Golden2- like transcription factor. Rx1 binds to NbGlk1 in vitro and in planta. NbGlk1 binds to known Golden2-like consensus DNA sequences. Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro. NbGlk1 activates cellular responses to potato virus X, whereas Rx1 associates with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX. This study provides new mechanistic insight into how an NLR can co-ordinate an immune signalling response at DNA following pathogen perception. ",

author = "Townsend, {Philip D.} and Dixon, {Christopher H.} and Slootweg, {Erik J.} and Sukarta, {Octavina C.A.} and Yang, {Ally W.H.} and Hughes, {Timothy R.} and Sharples, {Gary J.} and Lars-Olof Palsson and Takken, {Frank L.W.} and Aska Goverse and Cann, {Martin J.}",

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doi = "10.1074/jbc.RA117.000485",

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AU - Townsend, Philip D.

AU - Dixon, Christopher H.

AU - Slootweg, Erik J.

AU - Sukarta, Octavina C.A.

AU - Yang, Ally W.H.

AU - Hughes, Timothy R.

AU - Sharples, Gary J.

AU - Palsson, Lars-Olof

AU - Takken, Frank L.W.

AU - Goverse, Aska

AU - Cann, Martin J.

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N2 - Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato is further able to bind and distort double-stranded DNA. However, Rx1 host targets that support a role for Rx1 in transcriptional reprogramming at DNA are unknown. Here we report a functional interaction between Rx1 and NbGlk1, a Golden2- like transcription factor. Rx1 binds to NbGlk1 in vitro and in planta. NbGlk1 binds to known Golden2-like consensus DNA sequences. Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro. NbGlk1 activates cellular responses to potato virus X, whereas Rx1 associates with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX. This study provides new mechanistic insight into how an NLR can co-ordinate an immune signalling response at DNA following pathogen perception.

AB - Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato is further able to bind and distort double-stranded DNA. However, Rx1 host targets that support a role for Rx1 in transcriptional reprogramming at DNA are unknown. Here we report a functional interaction between Rx1 and NbGlk1, a Golden2- like transcription factor. Rx1 binds to NbGlk1 in vitro and in planta. NbGlk1 binds to known Golden2-like consensus DNA sequences. Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro. NbGlk1 activates cellular responses to potato virus X, whereas Rx1 associates with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX. This study provides new mechanistic insight into how an NLR can co-ordinate an immune signalling response at DNA following pathogen perception.

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DO - 10.1074/jbc.RA117.000485

M3 - Article

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VL - 293

SP - 3218

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 9

ER -

The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor

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