The influenza A virus NS1 protein binds small interfering RNAs and suppresses RNA silencing in plants

E.C. Bucher, J.C. Hemmes, P. de Haan, R.W. Goldbach, M.W. Prins

Research output: Contribution to journalArticleAcademicpeer-review

158 Citations (Scopus)

Abstract

RNA silencing comprises a set of sequence-specific RNA degradation pathways that occur in a wide range of eukaryotes, including animals, fungi and plants. A hallmark of RNA silencing is the presence of small interfering RNA molecules (siRNAs). The siRNAs are generated by cleavage of larger double-stranded RNAs (dsRNAs) and provide the sequence specificity for degradation of cognate RNA molecules. In plants, RNA silencing plays a key role in developmental processes and in control of virus replication. It has been shown that many plant viruses encode proteins, denoted RNA silencing suppressors, that interfere with this antiviral response. Although RNA silencing has been shown to occur in vertebrates, no relationship with inhibition of virus replication has been demonstrated to date. Here we show that the NS1 protein of human influenza A virus has an RNA silencing suppression activity in plants, similar to established RNA silencing suppressor proteins of plant viruses. In addition, NS1 was shown to be capable of binding siRNAs. The data presented here fit with a potential role for NS1 in counteracting innate antiviral responses in vertebrates by sequestering siRNAs.
Original languageEnglish
Pages (from-to)983-991
Number of pages9
JournalJournal of General Virology
Volume85
Issue number4
DOIs
Publication statusPublished - 2004

Keywords

  • double-stranded-rna
  • translation initiation-factor
  • nicotiana-benthamiana
  • messenger-rna
  • transgenic plants
  • gene-expression
  • coat protein
  • human-cells
  • c-elegans
  • targets

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