The influence of humic acids on the activities of lysozyme and urease

Yan Li; Wenfeng Tan; Luuk K. Koopal

doi:10.1007/978-94-007-5634-2_148

The influence of humic acids on the activities of lysozyme and urease

Yan Li, Wenfeng Tan^*, Luuk K. Koopal

^*Corresponding author for this work

Physical Chemistry and Soft Matter

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

To evaluate electrostatic interactions between humic acids and proteins and the effects of pH, ionic strength and mass ratios (HAs/enzymes) on activities of immobilized enzymes, soil humic acids (including HA and FA) and two enzymes (lysozyme and urease) were applied in our experiment. Electrostatic attraction and repulsion play important roles in their interaction, which is also pH dependent. Salt could screen not only electrostatic attraction but also repulsive force, which is dependent on the mixing fractions of HAs and enzymes. Two mechanisms were presented for the interaction of HAs with lysozyme and urease: encapsulated lysozyme into humic acid network structure to block the active sites or deformation of the tertiary structure of lysozyme and adsorbed urease onto humic acids to stabilize the enzyme structure.

Original language	English
Title of host publication	Functions of Natural Organic Matter in Changing Environment
Editors	J. Xu, J. Wu, Y. He
Publisher	Springer
Pages	817-821
Number of pages	5
ISBN (Electronic)	9789400756342
ISBN (Print)	9789400756335
DOIs	https://doi.org/10.1007/978-94-007-5634-2_148
Publication status	Published - 1 Aug 2013

Keywords

Activity
Fulvic acid (FA)
Humic acid (HA)
Lysozyme (LSZ)
Urease

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10.1007/978-94-007-5634-2_148

Cite this

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title = "The influence of humic acids on the activities of lysozyme and urease",

abstract = "To evaluate electrostatic interactions between humic acids and proteins and the effects of pH, ionic strength and mass ratios (HAs/enzymes) on activities of immobilized enzymes, soil humic acids (including HA and FA) and two enzymes (lysozyme and urease) were applied in our experiment. Electrostatic attraction and repulsion play important roles in their interaction, which is also pH dependent. Salt could screen not only electrostatic attraction but also repulsive force, which is dependent on the mixing fractions of HAs and enzymes. Two mechanisms were presented for the interaction of HAs with lysozyme and urease: encapsulated lysozyme into humic acid network structure to block the active sites or deformation of the tertiary structure of lysozyme and adsorbed urease onto humic acids to stabilize the enzyme structure.",

keywords = "Activity, Fulvic acid (FA), Humic acid (HA), Lysozyme (LSZ), Urease",

author = "Yan Li and Wenfeng Tan and Koopal, \{Luuk K.\}",

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doi = "10.1007/978-94-007-5634-2\_148",

language = "English",

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editor = "J. Xu and J. Wu and Y. He",

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TY - CHAP

T1 - The influence of humic acids on the activities of lysozyme and urease

AU - Li, Yan

AU - Tan, Wenfeng

AU - Koopal, Luuk K.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - To evaluate electrostatic interactions between humic acids and proteins and the effects of pH, ionic strength and mass ratios (HAs/enzymes) on activities of immobilized enzymes, soil humic acids (including HA and FA) and two enzymes (lysozyme and urease) were applied in our experiment. Electrostatic attraction and repulsion play important roles in their interaction, which is also pH dependent. Salt could screen not only electrostatic attraction but also repulsive force, which is dependent on the mixing fractions of HAs and enzymes. Two mechanisms were presented for the interaction of HAs with lysozyme and urease: encapsulated lysozyme into humic acid network structure to block the active sites or deformation of the tertiary structure of lysozyme and adsorbed urease onto humic acids to stabilize the enzyme structure.

AB - To evaluate electrostatic interactions between humic acids and proteins and the effects of pH, ionic strength and mass ratios (HAs/enzymes) on activities of immobilized enzymes, soil humic acids (including HA and FA) and two enzymes (lysozyme and urease) were applied in our experiment. Electrostatic attraction and repulsion play important roles in their interaction, which is also pH dependent. Salt could screen not only electrostatic attraction but also repulsive force, which is dependent on the mixing fractions of HAs and enzymes. Two mechanisms were presented for the interaction of HAs with lysozyme and urease: encapsulated lysozyme into humic acid network structure to block the active sites or deformation of the tertiary structure of lysozyme and adsorbed urease onto humic acids to stabilize the enzyme structure.

KW - Activity

KW - Fulvic acid (FA)

KW - Humic acid (HA)

KW - Lysozyme (LSZ)

KW - Urease

U2 - 10.1007/978-94-007-5634-2_148

DO - 10.1007/978-94-007-5634-2_148

M3 - Chapter

AN - SCOPUS:84932624367

SN - 9789400756335

SP - 817

EP - 821

BT - Functions of Natural Organic Matter in Changing Environment

A2 - Xu, J.

A2 - Wu, J.

A2 - He, Y.

PB - Springer

ER -

The influence of humic acids on the activities of lysozyme and urease

Abstract

Keywords

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