The influence of endometrium on ultrastructure and estrogen release of day-11 and day-13 pig blastocysts in vitro.

J. van der Meulen, F.A. Helmond, C.P. Oudenaarden, G. te Kronnie

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Day-11 spherical and Day-13 filamentous blastocysts were cultured in vitro without supplementation of oestrogen precursors for 2 consecutive periods of 24 h, with or without endometrial tissue. Spherical Day-11 blastocysts flushed from one uterine horn (5.5 /- 0.8 blastocysts; 5.3 /- 0.4 mm in diameter; mean /- s.d.) did not show gross morphological changes during the culture period. Free and conjugated oestrone (E1) and oestradiol-17 beta (E2) were released during the first and second 24 h culture period and was maximal during the first 24 h. In the presence of endometrial tissue the release of oestrogens from the blastocysts was reduced to 30-50%. Clewed Day-13 filamentous blastocysts, recovered after flushing, formed trophospheres and also in these cultures both free and conjugated E1 and E2 were released. In this case the addition of endometrial tissue did not affect the release of oestrogens from the blastocysts. Single Day-13 filamentous blastocysts cultured without endometrial tissue (n = 33, from 4 gilts), developed trophospheres and all blastocysts released E1 and E2. During the first 24 h on average per blastocyst 2.6 ng E1 (range 1.6-4.0) and 9.7 ng E2 (range 4.3-15.3) were released. In all cultures of spherical and filamentous blastocysts E2 release exceeded E1 release.These data show that Day 11-13 blastocysts are able to release both free and conjugated E1 and E2 during in vitro culture from endogenous precursors. The addition of endometrial tissue reduced the release of oestrogens from Day-11 but not Day-13 blastocysts. Whether this is due to an altered oestrogen release of the blastocysts in the presence of endometrium on Day-11 or due, to an increased uptake of oestrogens by the endometrium on Day-11 remains to be investigated.
Original languageEnglish
Pages (from-to)225-234
JournalNetherlands Journal of Agricultural Science
Volume42
Publication statusPublished - 1994

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endometrium
Blastocyst
Endometrium
blastocyst
estrogens
ultrastructure
Estrogens
Swine
swine
In Vitro Techniques
estrone
Estrone
gilts
in vitro culture
estradiol
Estradiol

Cite this

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title = "The influence of endometrium on ultrastructure and estrogen release of day-11 and day-13 pig blastocysts in vitro.",
abstract = "Day-11 spherical and Day-13 filamentous blastocysts were cultured in vitro without supplementation of oestrogen precursors for 2 consecutive periods of 24 h, with or without endometrial tissue. Spherical Day-11 blastocysts flushed from one uterine horn (5.5 /- 0.8 blastocysts; 5.3 /- 0.4 mm in diameter; mean /- s.d.) did not show gross morphological changes during the culture period. Free and conjugated oestrone (E1) and oestradiol-17 beta (E2) were released during the first and second 24 h culture period and was maximal during the first 24 h. In the presence of endometrial tissue the release of oestrogens from the blastocysts was reduced to 30-50{\%}. Clewed Day-13 filamentous blastocysts, recovered after flushing, formed trophospheres and also in these cultures both free and conjugated E1 and E2 were released. In this case the addition of endometrial tissue did not affect the release of oestrogens from the blastocysts. Single Day-13 filamentous blastocysts cultured without endometrial tissue (n = 33, from 4 gilts), developed trophospheres and all blastocysts released E1 and E2. During the first 24 h on average per blastocyst 2.6 ng E1 (range 1.6-4.0) and 9.7 ng E2 (range 4.3-15.3) were released. In all cultures of spherical and filamentous blastocysts E2 release exceeded E1 release.These data show that Day 11-13 blastocysts are able to release both free and conjugated E1 and E2 during in vitro culture from endogenous precursors. The addition of endometrial tissue reduced the release of oestrogens from Day-11 but not Day-13 blastocysts. Whether this is due to an altered oestrogen release of the blastocysts in the presence of endometrium on Day-11 or due, to an increased uptake of oestrogens by the endometrium on Day-11 remains to be investigated.",
author = "{van der Meulen}, J. and F.A. Helmond and C.P. Oudenaarden and {te Kronnie}, G.",
year = "1994",
language = "English",
volume = "42",
pages = "225--234",
journal = "Netherlands Journal of Agricultural Science",
issn = "0028-2928",
publisher = "Koninklijk Landbouwkundige Vereniging/Royal Netherlands Society of Agricultural Sciences",

}

The influence of endometrium on ultrastructure and estrogen release of day-11 and day-13 pig blastocysts in vitro. / van der Meulen, J.; Helmond, F.A.; Oudenaarden, C.P.; te Kronnie, G.

In: Netherlands Journal of Agricultural Science, Vol. 42, 1994, p. 225-234.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The influence of endometrium on ultrastructure and estrogen release of day-11 and day-13 pig blastocysts in vitro.

AU - van der Meulen, J.

AU - Helmond, F.A.

AU - Oudenaarden, C.P.

AU - te Kronnie, G.

PY - 1994

Y1 - 1994

N2 - Day-11 spherical and Day-13 filamentous blastocysts were cultured in vitro without supplementation of oestrogen precursors for 2 consecutive periods of 24 h, with or without endometrial tissue. Spherical Day-11 blastocysts flushed from one uterine horn (5.5 /- 0.8 blastocysts; 5.3 /- 0.4 mm in diameter; mean /- s.d.) did not show gross morphological changes during the culture period. Free and conjugated oestrone (E1) and oestradiol-17 beta (E2) were released during the first and second 24 h culture period and was maximal during the first 24 h. In the presence of endometrial tissue the release of oestrogens from the blastocysts was reduced to 30-50%. Clewed Day-13 filamentous blastocysts, recovered after flushing, formed trophospheres and also in these cultures both free and conjugated E1 and E2 were released. In this case the addition of endometrial tissue did not affect the release of oestrogens from the blastocysts. Single Day-13 filamentous blastocysts cultured without endometrial tissue (n = 33, from 4 gilts), developed trophospheres and all blastocysts released E1 and E2. During the first 24 h on average per blastocyst 2.6 ng E1 (range 1.6-4.0) and 9.7 ng E2 (range 4.3-15.3) were released. In all cultures of spherical and filamentous blastocysts E2 release exceeded E1 release.These data show that Day 11-13 blastocysts are able to release both free and conjugated E1 and E2 during in vitro culture from endogenous precursors. The addition of endometrial tissue reduced the release of oestrogens from Day-11 but not Day-13 blastocysts. Whether this is due to an altered oestrogen release of the blastocysts in the presence of endometrium on Day-11 or due, to an increased uptake of oestrogens by the endometrium on Day-11 remains to be investigated.

AB - Day-11 spherical and Day-13 filamentous blastocysts were cultured in vitro without supplementation of oestrogen precursors for 2 consecutive periods of 24 h, with or without endometrial tissue. Spherical Day-11 blastocysts flushed from one uterine horn (5.5 /- 0.8 blastocysts; 5.3 /- 0.4 mm in diameter; mean /- s.d.) did not show gross morphological changes during the culture period. Free and conjugated oestrone (E1) and oestradiol-17 beta (E2) were released during the first and second 24 h culture period and was maximal during the first 24 h. In the presence of endometrial tissue the release of oestrogens from the blastocysts was reduced to 30-50%. Clewed Day-13 filamentous blastocysts, recovered after flushing, formed trophospheres and also in these cultures both free and conjugated E1 and E2 were released. In this case the addition of endometrial tissue did not affect the release of oestrogens from the blastocysts. Single Day-13 filamentous blastocysts cultured without endometrial tissue (n = 33, from 4 gilts), developed trophospheres and all blastocysts released E1 and E2. During the first 24 h on average per blastocyst 2.6 ng E1 (range 1.6-4.0) and 9.7 ng E2 (range 4.3-15.3) were released. In all cultures of spherical and filamentous blastocysts E2 release exceeded E1 release.These data show that Day 11-13 blastocysts are able to release both free and conjugated E1 and E2 during in vitro culture from endogenous precursors. The addition of endometrial tissue reduced the release of oestrogens from Day-11 but not Day-13 blastocysts. Whether this is due to an altered oestrogen release of the blastocysts in the presence of endometrium on Day-11 or due, to an increased uptake of oestrogens by the endometrium on Day-11 remains to be investigated.

M3 - Article

VL - 42

SP - 225

EP - 234

JO - Netherlands Journal of Agricultural Science

JF - Netherlands Journal of Agricultural Science

SN - 0028-2928

ER -