The influence of breast milk and infant formulae hydrolysates on bacterial adhesion and Caco-2 cells functioning

Ewa Fiedorowicz, Lidia Hanna Markiewicz, Katarzyna Sidor, Dominika Świątecka, Anna Cieślińska, Michał Matysiewicz, Krystyna Piskorz-Ogórek, Edyta Sienkiewicz-Szłapka, Gosia Teodorowicz, Aleksander Świątecki, Elżbieta Kostyra*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

The aim of the study was to determine the concentration of BCM7 in human milk and infant formulae (IF) before and after eznymatic hydrolysis, and to evaluate the effect of obtained hydrolysates on interleukin-8 (IL-8) secretion and on proliferation of enterocytes in the in vitro model (Caco-2 cells). This study evaluates also the effect of hydrolysates on the adhesion of intestinal microbiota isolated from faeces of both healthy (H) and allergic (A) infants. In the study we investigated breast milk delivered by mothers of healthy (‘healthy milk’ HM) and allergic (‘allergic milk’ AM) infants. Three infant formulae were investigated: from hydrolysed cow casein (IF1), from hydrolysed cow whey (IF2) and from whole cow milk (IF3). Intestinal bacteria: Bifidobacterium, lactic acid bacteria, Enterobacteriaceae, Clostridium and Enterococcus were isolated from faeces of five healthy and five allergic infants. Mixtures of bacterial isolates and bacteria adhering to Caco-2 cells were characterised qualitatively with PCR-DGGE, and quantitavely with FISH. Concentration of BCM7 in breast milk and infant formulae was 1.6 to 8.9 times higher after enzymatic hydrolysis in comparison to undigested samples. The presence of this peptide resulted in alteration of intestinal epithelial proliferation and increase in secretion of IL-8. The quantitative profile of adherred bacteria applied as a mix of all isolates from healthy infants (H-MIX) was unchanged in the presence of HM hydrolysate and was modulated (increased number of beneficial Bifidobacterium and reduced commensal Enterobacteriaceae) in the presence of all IF hydrolysates. The presence of IF hydrolysates affected the profile of adhering isolates obtained from allergic infants (A-MIX) and reduced the adhesion of Enterobacteriaceae; the IF2 and IF3 hydrolysates decreased also the total number of adhering bacteria (TBN). However, a stimulating effect of AM hydrolysate on A-MIX adhesion (increased TBN) was observed.

Original languageEnglish
Pages (from-to)679-688
JournalFood Research International
Volume89
DOIs
Publication statusPublished - 2016

Fingerprint

Bacterial Adhesion
bacterial adhesion
Infant Formula
Caco-2 Cells
infant formulas
Human Milk
breast milk
hydrolysates
Bacteria
Enterobacteriaceae
Milk
Bifidobacterium
Interleukin-8
Feces
adhesion
cells
Hydrolysis
interleukin-8
intestinal microorganisms
milk

Keywords

  • Bacterial adhesion
  • Breast milk
  • Food allergy
  • Infant formula
  • β-casomorphin-7

Cite this

Fiedorowicz, E., Markiewicz, L. H., Sidor, K., Świątecka, D., Cieślińska, A., Matysiewicz, M., ... Kostyra, E. (2016). The influence of breast milk and infant formulae hydrolysates on bacterial adhesion and Caco-2 cells functioning. Food Research International, 89, 679-688. https://doi.org/10.1016/j.foodres.2016.09.022
Fiedorowicz, Ewa ; Markiewicz, Lidia Hanna ; Sidor, Katarzyna ; Świątecka, Dominika ; Cieślińska, Anna ; Matysiewicz, Michał ; Piskorz-Ogórek, Krystyna ; Sienkiewicz-Szłapka, Edyta ; Teodorowicz, Gosia ; Świątecki, Aleksander ; Kostyra, Elżbieta. / The influence of breast milk and infant formulae hydrolysates on bacterial adhesion and Caco-2 cells functioning. In: Food Research International. 2016 ; Vol. 89. pp. 679-688.
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abstract = "The aim of the study was to determine the concentration of BCM7 in human milk and infant formulae (IF) before and after eznymatic hydrolysis, and to evaluate the effect of obtained hydrolysates on interleukin-8 (IL-8) secretion and on proliferation of enterocytes in the in vitro model (Caco-2 cells). This study evaluates also the effect of hydrolysates on the adhesion of intestinal microbiota isolated from faeces of both healthy (H) and allergic (A) infants. In the study we investigated breast milk delivered by mothers of healthy (‘healthy milk’ HM) and allergic (‘allergic milk’ AM) infants. Three infant formulae were investigated: from hydrolysed cow casein (IF1), from hydrolysed cow whey (IF2) and from whole cow milk (IF3). Intestinal bacteria: Bifidobacterium, lactic acid bacteria, Enterobacteriaceae, Clostridium and Enterococcus were isolated from faeces of five healthy and five allergic infants. Mixtures of bacterial isolates and bacteria adhering to Caco-2 cells were characterised qualitatively with PCR-DGGE, and quantitavely with FISH. Concentration of BCM7 in breast milk and infant formulae was 1.6 to 8.9 times higher after enzymatic hydrolysis in comparison to undigested samples. The presence of this peptide resulted in alteration of intestinal epithelial proliferation and increase in secretion of IL-8. The quantitative profile of adherred bacteria applied as a mix of all isolates from healthy infants (H-MIX) was unchanged in the presence of HM hydrolysate and was modulated (increased number of beneficial Bifidobacterium and reduced commensal Enterobacteriaceae) in the presence of all IF hydrolysates. The presence of IF hydrolysates affected the profile of adhering isolates obtained from allergic infants (A-MIX) and reduced the adhesion of Enterobacteriaceae; the IF2 and IF3 hydrolysates decreased also the total number of adhering bacteria (TBN). However, a stimulating effect of AM hydrolysate on A-MIX adhesion (increased TBN) was observed.",
keywords = "Bacterial adhesion, Breast milk, Food allergy, Infant formula, β-casomorphin-7",
author = "Ewa Fiedorowicz and Markiewicz, {Lidia Hanna} and Katarzyna Sidor and Dominika Świątecka and Anna Cieślińska and Michał Matysiewicz and Krystyna Piskorz-Og{\'o}rek and Edyta Sienkiewicz-Szłapka and Gosia Teodorowicz and Aleksander Świątecki and Elżbieta Kostyra",
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Fiedorowicz, E, Markiewicz, LH, Sidor, K, Świątecka, D, Cieślińska, A, Matysiewicz, M, Piskorz-Ogórek, K, Sienkiewicz-Szłapka, E, Teodorowicz, G, Świątecki, A & Kostyra, E 2016, 'The influence of breast milk and infant formulae hydrolysates on bacterial adhesion and Caco-2 cells functioning', Food Research International, vol. 89, pp. 679-688. https://doi.org/10.1016/j.foodres.2016.09.022

The influence of breast milk and infant formulae hydrolysates on bacterial adhesion and Caco-2 cells functioning. / Fiedorowicz, Ewa; Markiewicz, Lidia Hanna; Sidor, Katarzyna; Świątecka, Dominika; Cieślińska, Anna; Matysiewicz, Michał; Piskorz-Ogórek, Krystyna; Sienkiewicz-Szłapka, Edyta; Teodorowicz, Gosia; Świątecki, Aleksander; Kostyra, Elżbieta.

In: Food Research International, Vol. 89, 2016, p. 679-688.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The influence of breast milk and infant formulae hydrolysates on bacterial adhesion and Caco-2 cells functioning

AU - Fiedorowicz, Ewa

AU - Markiewicz, Lidia Hanna

AU - Sidor, Katarzyna

AU - Świątecka, Dominika

AU - Cieślińska, Anna

AU - Matysiewicz, Michał

AU - Piskorz-Ogórek, Krystyna

AU - Sienkiewicz-Szłapka, Edyta

AU - Teodorowicz, Gosia

AU - Świątecki, Aleksander

AU - Kostyra, Elżbieta

PY - 2016

Y1 - 2016

N2 - The aim of the study was to determine the concentration of BCM7 in human milk and infant formulae (IF) before and after eznymatic hydrolysis, and to evaluate the effect of obtained hydrolysates on interleukin-8 (IL-8) secretion and on proliferation of enterocytes in the in vitro model (Caco-2 cells). This study evaluates also the effect of hydrolysates on the adhesion of intestinal microbiota isolated from faeces of both healthy (H) and allergic (A) infants. In the study we investigated breast milk delivered by mothers of healthy (‘healthy milk’ HM) and allergic (‘allergic milk’ AM) infants. Three infant formulae were investigated: from hydrolysed cow casein (IF1), from hydrolysed cow whey (IF2) and from whole cow milk (IF3). Intestinal bacteria: Bifidobacterium, lactic acid bacteria, Enterobacteriaceae, Clostridium and Enterococcus were isolated from faeces of five healthy and five allergic infants. Mixtures of bacterial isolates and bacteria adhering to Caco-2 cells were characterised qualitatively with PCR-DGGE, and quantitavely with FISH. Concentration of BCM7 in breast milk and infant formulae was 1.6 to 8.9 times higher after enzymatic hydrolysis in comparison to undigested samples. The presence of this peptide resulted in alteration of intestinal epithelial proliferation and increase in secretion of IL-8. The quantitative profile of adherred bacteria applied as a mix of all isolates from healthy infants (H-MIX) was unchanged in the presence of HM hydrolysate and was modulated (increased number of beneficial Bifidobacterium and reduced commensal Enterobacteriaceae) in the presence of all IF hydrolysates. The presence of IF hydrolysates affected the profile of adhering isolates obtained from allergic infants (A-MIX) and reduced the adhesion of Enterobacteriaceae; the IF2 and IF3 hydrolysates decreased also the total number of adhering bacteria (TBN). However, a stimulating effect of AM hydrolysate on A-MIX adhesion (increased TBN) was observed.

AB - The aim of the study was to determine the concentration of BCM7 in human milk and infant formulae (IF) before and after eznymatic hydrolysis, and to evaluate the effect of obtained hydrolysates on interleukin-8 (IL-8) secretion and on proliferation of enterocytes in the in vitro model (Caco-2 cells). This study evaluates also the effect of hydrolysates on the adhesion of intestinal microbiota isolated from faeces of both healthy (H) and allergic (A) infants. In the study we investigated breast milk delivered by mothers of healthy (‘healthy milk’ HM) and allergic (‘allergic milk’ AM) infants. Three infant formulae were investigated: from hydrolysed cow casein (IF1), from hydrolysed cow whey (IF2) and from whole cow milk (IF3). Intestinal bacteria: Bifidobacterium, lactic acid bacteria, Enterobacteriaceae, Clostridium and Enterococcus were isolated from faeces of five healthy and five allergic infants. Mixtures of bacterial isolates and bacteria adhering to Caco-2 cells were characterised qualitatively with PCR-DGGE, and quantitavely with FISH. Concentration of BCM7 in breast milk and infant formulae was 1.6 to 8.9 times higher after enzymatic hydrolysis in comparison to undigested samples. The presence of this peptide resulted in alteration of intestinal epithelial proliferation and increase in secretion of IL-8. The quantitative profile of adherred bacteria applied as a mix of all isolates from healthy infants (H-MIX) was unchanged in the presence of HM hydrolysate and was modulated (increased number of beneficial Bifidobacterium and reduced commensal Enterobacteriaceae) in the presence of all IF hydrolysates. The presence of IF hydrolysates affected the profile of adhering isolates obtained from allergic infants (A-MIX) and reduced the adhesion of Enterobacteriaceae; the IF2 and IF3 hydrolysates decreased also the total number of adhering bacteria (TBN). However, a stimulating effect of AM hydrolysate on A-MIX adhesion (increased TBN) was observed.

KW - Bacterial adhesion

KW - Breast milk

KW - Food allergy

KW - Infant formula

KW - β-casomorphin-7

U2 - 10.1016/j.foodres.2016.09.022

DO - 10.1016/j.foodres.2016.09.022

M3 - Article

VL - 89

SP - 679

EP - 688

JO - Food Research International

JF - Food Research International

SN - 0963-9969

ER -