The importance of a sterile rhizosphere when phenotyping for root exudation

R.C.P. Kuijken, J.F.H. Snel, M.M. Heddes, H.J. Bouwmeester, L.F.M. Marcelis

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Background and aims A fast and reliable phenotyping system forms a major bottleneck in root exudation research. Our goal was to develop such a system to quantify genetic and environmental effects on root exudation. Another aim was characterizing non-sterile phenotyping. Methods We developed a system in which plants can be grown with non-sterile shoot environments and sterile rhizospheres. These sterile systems were compared with non-sterile controls. Results In non-sterile rhizospheres exogenous carbon disappeared quickly with a half-life of 2 to 3 h and root exudate concentrations remained below detection limit. In sterile rhizospheres exogenous carbon levels were relatively stable or depleted slower than in non-sterile rhizospheres and organic acid build-up occurred. Tomato (Solanum lycopersicum) could be grown with sterile roots for several months. Conclusions The differences in carbon depletion in sterile and non-sterile rhizospheres was most likely due to the absence of microbial catabolism in sterile rhizospheres. These results prove that using a sterile phenotyping system is essential to study the quantity and composition of root exudates. The sterile system described in this paper eliminates the obscuring effect caused by microbes on exudation levels. It offers a stable, reliable and easy phenotyping method and can be used to investigate genetic and environmental effects on exudation.
Original languageEnglish
Pages (from-to)131-142
JournalPlant and Soil
Volume387
Issue number1-2
DOIs
Publication statusPublished - 2015

Keywords

  • lupinus-albus l.
  • oryza-sativa l.
  • zea-mays l
  • organic-acids
  • phosphorus deficiency
  • aluminum resistance
  • white lupin
  • carboxylate exudation
  • malate transporter
  • nutrient solution

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