The impact of rearing environment on the development of gut microbiota in tilapia larvae

Christos Giatsis*, Detmer Sipkema, Hauke Smidt, Hans Heilig, Giulia Benvenuti, Johan Verreth, Marc Verdegem

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

60 Citations (Scopus)

Abstract

This study explores the effect of rearing environment on water bacterial communities (BC) and the association with those present in the gut of Nile tilapia larvae (Oreochromis niloticus, Linnaeus) grown in either recirculating or active suspension systems. 454 pyrosequencing of PCR-Amplified 16S rRNA gene fragments was applied to characterize the composition of water, feed and gut bacteria communities. Observed changes in water BC over time and differences in water BCs between systems were highly correlated with corresponding water physico-chemical properties. Differences in gut bacterial communities during larval development were correlated with differences in water communities between systems. The correlation of feed BC with those in the gut was minor compared to that between gut and water, reflected by the fact that 4 to 43 times more OTUs were shared between water and gut than between gut and feed BC. Shared OTUs between water and gut suggest a successful transfer of microorganisms from water into the gut, and give insight about the niche and ecological adaptability of water microorganisms inside the gut. These findings suggest that steering of gut microbial communities could be possible through water microbial management derived by the design and functionality of the rearing system.

Original languageEnglish
Article number18206
Number of pages15
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 11 Dec 2015

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Tilapia (Cichlidae)
intestinal microorganisms
rearing
digestive system
larvae
bacterial communities
water
Otus
Oreochromis niloticus
suspension systems
microorganisms
hydrochemistry
larval development
microbial communities
physicochemical properties
niches

Cite this

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title = "The impact of rearing environment on the development of gut microbiota in tilapia larvae",
abstract = "This study explores the effect of rearing environment on water bacterial communities (BC) and the association with those present in the gut of Nile tilapia larvae (Oreochromis niloticus, Linnaeus) grown in either recirculating or active suspension systems. 454 pyrosequencing of PCR-Amplified 16S rRNA gene fragments was applied to characterize the composition of water, feed and gut bacteria communities. Observed changes in water BC over time and differences in water BCs between systems were highly correlated with corresponding water physico-chemical properties. Differences in gut bacterial communities during larval development were correlated with differences in water communities between systems. The correlation of feed BC with those in the gut was minor compared to that between gut and water, reflected by the fact that 4 to 43 times more OTUs were shared between water and gut than between gut and feed BC. Shared OTUs between water and gut suggest a successful transfer of microorganisms from water into the gut, and give insight about the niche and ecological adaptability of water microorganisms inside the gut. These findings suggest that steering of gut microbial communities could be possible through water microbial management derived by the design and functionality of the rearing system.",
author = "Christos Giatsis and Detmer Sipkema and Hauke Smidt and Hans Heilig and Giulia Benvenuti and Johan Verreth and Marc Verdegem",
year = "2015",
month = "12",
day = "11",
doi = "10.1038/srep18206",
language = "English",
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journal = "Scientific Reports",
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The impact of rearing environment on the development of gut microbiota in tilapia larvae. / Giatsis, Christos; Sipkema, Detmer; Smidt, Hauke; Heilig, Hans; Benvenuti, Giulia; Verreth, Johan; Verdegem, Marc.

In: Scientific Reports, Vol. 5, 18206, 11.12.2015.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Giatsis, Christos

AU - Sipkema, Detmer

AU - Smidt, Hauke

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AU - Benvenuti, Giulia

AU - Verreth, Johan

AU - Verdegem, Marc

PY - 2015/12/11

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AB - This study explores the effect of rearing environment on water bacterial communities (BC) and the association with those present in the gut of Nile tilapia larvae (Oreochromis niloticus, Linnaeus) grown in either recirculating or active suspension systems. 454 pyrosequencing of PCR-Amplified 16S rRNA gene fragments was applied to characterize the composition of water, feed and gut bacteria communities. Observed changes in water BC over time and differences in water BCs between systems were highly correlated with corresponding water physico-chemical properties. Differences in gut bacterial communities during larval development were correlated with differences in water communities between systems. The correlation of feed BC with those in the gut was minor compared to that between gut and water, reflected by the fact that 4 to 43 times more OTUs were shared between water and gut than between gut and feed BC. Shared OTUs between water and gut suggest a successful transfer of microorganisms from water into the gut, and give insight about the niche and ecological adaptability of water microorganisms inside the gut. These findings suggest that steering of gut microbial communities could be possible through water microbial management derived by the design and functionality of the rearing system.

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