The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly

Ellen Wilms*, Daisy M.A.E. Jonkers, Huub F.J. Savelkoul, Montserrat Elizalde, Lea Tischmann, Paul de Vos, Ad A.M. Masclee, Freddy J. Troost

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.

Original languageEnglish
Article number1554
JournalNutrients
Volume11
Issue number7
DOIs
Publication statusPublished - 1 Jul 2019

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young adults
pectins
Young Adult
placebos
Placebos
Permeability
permeability
Age Groups
electrical resistance
Sigmoid Colon
fluorescein
pectin
Fluorescein
Electric Impedance
Immunoglobulin A
Genes
biopsy
genes
digestive system
transcription (genetics)

Keywords

  • Aging
  • Defense
  • Dietary fiber
  • Gastrointestinal
  • Intestinal permeability
  • Tight junctions
  • Tolerance

Cite this

Wilms, E., Jonkers, D. M. A. E., Savelkoul, H. F. J., Elizalde, M., Tischmann, L., de Vos, P., ... Troost, F. J. (2019). The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly. Nutrients, 11(7), [1554]. https://doi.org/10.3390/nu11071554
Wilms, Ellen ; Jonkers, Daisy M.A.E. ; Savelkoul, Huub F.J. ; Elizalde, Montserrat ; Tischmann, Lea ; de Vos, Paul ; Masclee, Ad A.M. ; Troost, Freddy J. / The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly. In: Nutrients. 2019 ; Vol. 11, No. 7.
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abstract = "Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.",
keywords = "Aging, Defense, Dietary fiber, Gastrointestinal, Intestinal permeability, Tight junctions, Tolerance",
author = "Ellen Wilms and Jonkers, {Daisy M.A.E.} and Savelkoul, {Huub F.J.} and Montserrat Elizalde and Lea Tischmann and {de Vos}, Paul and Masclee, {Ad A.M.} and Troost, {Freddy J.}",
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Wilms, E, Jonkers, DMAE, Savelkoul, HFJ, Elizalde, M, Tischmann, L, de Vos, P, Masclee, AAM & Troost, FJ 2019, 'The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly', Nutrients, vol. 11, no. 7, 1554. https://doi.org/10.3390/nu11071554

The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly. / Wilms, Ellen; Jonkers, Daisy M.A.E.; Savelkoul, Huub F.J.; Elizalde, Montserrat; Tischmann, Lea; de Vos, Paul; Masclee, Ad A.M.; Troost, Freddy J.

In: Nutrients, Vol. 11, No. 7, 1554, 01.07.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly

AU - Wilms, Ellen

AU - Jonkers, Daisy M.A.E.

AU - Savelkoul, Huub F.J.

AU - Elizalde, Montserrat

AU - Tischmann, Lea

AU - de Vos, Paul

AU - Masclee, Ad A.M.

AU - Troost, Freddy J.

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.

AB - Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.

KW - Aging

KW - Defense

KW - Dietary fiber

KW - Gastrointestinal

KW - Intestinal permeability

KW - Tight junctions

KW - Tolerance

U2 - 10.3390/nu11071554

DO - 10.3390/nu11071554

M3 - Article

VL - 11

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 7

M1 - 1554

ER -