Abstract
Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.
| Original language | English |
|---|---|
| Article number | 1554 |
| Journal | Nutrients |
| Volume | 11 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Jul 2019 |
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Keywords
- Aging
- Defense
- Dietary fiber
- Gastrointestinal
- Intestinal permeability
- Tight junctions
- Tolerance
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The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly. / Wilms, Ellen; Jonkers, Daisy M.A.E.; Savelkoul, Huub F.J.; Elizalde, Montserrat; Tischmann, Lea; de Vos, Paul; Masclee, Ad A.M.; Troost, Freddy J.
In: Nutrients, Vol. 11, No. 7, 1554, 01.07.2019.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly
AU - Wilms, Ellen
AU - Jonkers, Daisy M.A.E.
AU - Savelkoul, Huub F.J.
AU - Elizalde, Montserrat
AU - Tischmann, Lea
AU - de Vos, Paul
AU - Masclee, Ad A.M.
AU - Troost, Freddy J.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.
AB - Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.
KW - Aging
KW - Defense
KW - Dietary fiber
KW - Gastrointestinal
KW - Intestinal permeability
KW - Tight junctions
KW - Tolerance
U2 - 10.3390/nu11071554
DO - 10.3390/nu11071554
M3 - Article
VL - 11
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 7
M1 - 1554
ER -