Abstract
The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro–cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell
clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo
development in culture impinge on this HDAC-dependent pathway.
Original language | English |
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Pages (from-to) | 195-209 |
Number of pages | 15 |
Journal | The Plant Cell |
Volume | 26 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- brassica-napus l
- polycomb-group proteins
- plant-cell cycle
- somatic embryogenesis
- arabidopsis-thaliana
- embryo development
- retinoblastoma protein
- in-vitro
- microspore embryogenesis
- pollen embryogenesis
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Dive into the research topics of 'The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte'. Together they form a unique fingerprint.Datasets
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The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in Cultured Pollen
Li, H. (Creator), Soriano, M. (Creator), Cordewener, J. H. G. (Creator), Muino Acuna, J. M. (Creator), Fukuoka, H. (Creator), Angenent, G. C. (Creator) & Boutilier, K. A. (Creator), Plant Research International, 1 Jan 2016
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE49070
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