The fungal H+-ATPase from Neurospora crassa reconstituted with fusicoccin receptors senses fusicoccin signal

Mauro Marra, Alessandro Ballio*, Patrizia Battirossi, Vincenzo Fogliano, Maria Rosaria Fullone, Clifford L. Slayman, Patrizia Aducci

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Fusicoccin affects several physiological processes regulated by the plasma membrane H+-ATPase in higher plants while other organisms having P-type H+- ATPases (e.g., fungi) are fusicoccin-insensitive. We have previously shown that fusicoccin binding to its receptor is necessary for H+-ATPase stimulation and have achieved the functional reconstitution into liposomes of fusicoccin receptors and the H+-ATPase from maize. In this paper we show that fusicoccin sensitivity can be conferred on the H+-ATPase from Neurospora crassa, a fungus insensitive to fusicoccin. In fact, H+ pumping by purified H+-ATPase from Neurospora crassa reconstituted into liposomes containing crude or partially purified fusicoccin receptors from maize was markedly enhanced by fusicoccin. The stimulation of H+ pumping by fusicoccin is dependent upon pH, fusicoccin, and protein concentration, as was reported for the system reconstituted with both proteins from maize.

Original languageEnglish
Pages (from-to)1599-1603
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number5
DOIs
Publication statusPublished - 28 Feb 1995
Externally publishedYes

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