The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp.nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum

F.A.M. de Bok, H.J. Harmsen, C.M. Plugge, M.C. de Vries, A.D.L. Akkermans, W.M. de Vos, A.J.M. Stams

Research output: Contribution to journalArticleAcademicpeer-review

102 Citations (Scopus)

Abstract

A Gram-positive, spore-forming, syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov. strain HHT, was isolated as a co-culture with Methanospirillum hungatei JF-1T from anaerobic, freeze-dried granular sludge obtained from an upflow anaerobic sludge bed reactor treating sugar beet wastewater. The bacterium converted propionate to acetate in co-culture with Methanospirillum hungatei JF-1T or Methanobacterium formicicum MFNT, but not in co-culture with Methanobrevibacter arboriphilus AZ. The organism could not be cultured axenically with any of the substrates tested and therefore can be considered as a (the first) true anaerobic syntrophic bacterium. The bacterium contained two distinct 16S rRNA gene sequences, with 96·8 % sequence similarity, which were both expressed during syntrophic growth on propionate as revealed by fluorescent in situ hybridization. The most closely related organisms are Cryptanaerobacter phenolicus LR7.2T, a bacterium that transforms phenol into benzoate, and Pelotomaculum thermopropionicum SIT, a thermophilic, syntrophic propionate-oxidizing bacterium. Other related species belong to the Gram-positive, sulfate-reducing genus Desulfotomaculum. The type strain of Pelotomaculum schinkii is strain HHT (=ATCC BAA-615T=DSM 15200T)
Original languageEnglish
Pages (from-to)1697-1703
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume55
Issue number4
DOIs
Publication statusPublished - 2005

Keywords

  • gradient gel-electrophoresis
  • syntrophobacter-wolinii
  • phylogenetic analysis
  • smithella-propionica
  • granular sludge
  • methanogens
  • oxidation
  • pathway
  • anaerobe
  • sulfate

Fingerprint Dive into the research topics of 'The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp.nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum'. Together they form a unique fingerprint.

Cite this