TY - JOUR
T1 - The first report on the occurrence race 9 of the tomato leaf mold pathogen Cladosporium fulvum (syn. Passalora fulva) in Cuba
AU - Bernal-Cabrera, Alexander
AU - Martínez-Coca, Benedicto
AU - Herrera-Isla, Lidcay
AU - Ynfante-Martínez, Danay
AU - Peteira-Delgado, Belkis
AU - Portal, Orelvis
AU - Leiva-Mora, Michel
AU - de Wit, Pierre J.G.M.
PY - 2021
Y1 - 2021
N2 - Leaf mold caused by the fungus Cladosporium fulvum (syn. Passalora fulva) has been a serious disease in greenhouse tomatoes in Cuba, but less damage is seen in outdoor grown plants. This study was conducted to identify races of the causal agent of tomato leaf mold. A total of 36 single conidial isolates obtained from different provinces in Cuba and the reference strain race 0 were inoculated on a differential set of tomato cultivars carrying different Cf resistance genes. Further, a polymerase chain reaction (PCR) was developed using Avr9 gene-specific primers. As result, all isolates could overcome the Cf-9 resistance gene only, but not resistance genes Cf-2,Cf-4 and Cf-5 and must therefore be coined race 9. Race identity was also confirmed by PCR analysis, which showed that the Avr9 gene was not amplified in any of 36 single conidial isolates studied proving that they all lack this gene. This is the first report of the occurrence of race 9 of C. fulvum in Cuba. Pyramiding other Cf genes in hybrid of tomato may provide a more durable level of resistance to C. fulvum.
AB - Leaf mold caused by the fungus Cladosporium fulvum (syn. Passalora fulva) has been a serious disease in greenhouse tomatoes in Cuba, but less damage is seen in outdoor grown plants. This study was conducted to identify races of the causal agent of tomato leaf mold. A total of 36 single conidial isolates obtained from different provinces in Cuba and the reference strain race 0 were inoculated on a differential set of tomato cultivars carrying different Cf resistance genes. Further, a polymerase chain reaction (PCR) was developed using Avr9 gene-specific primers. As result, all isolates could overcome the Cf-9 resistance gene only, but not resistance genes Cf-2,Cf-4 and Cf-5 and must therefore be coined race 9. Race identity was also confirmed by PCR analysis, which showed that the Avr9 gene was not amplified in any of 36 single conidial isolates studied proving that they all lack this gene. This is the first report of the occurrence of race 9 of C. fulvum in Cuba. Pyramiding other Cf genes in hybrid of tomato may provide a more durable level of resistance to C. fulvum.
KW - Avr9 gene
KW - Cf resistance genes
KW - Race
KW - Tomato leaf mold
U2 - 10.1007/s10658-021-02261-4
DO - 10.1007/s10658-021-02261-4
M3 - Article
AN - SCOPUS:85103183742
SN - 0929-1873
VL - 160
SP - 731
EP - 736
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
IS - 3
ER -