The ferulic acid esterases of Chrysosporium lucknowense C1: Purification, characterization and their potential application in biorefinery

S. Kuhnel, L.A.M. Pouvreau, M.M. Appeldoorn, S.W.A. Hinz, H.A. Schols, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

Three ferulic acid esterases from the filamentous fungus Chrysosporium lucknowense C1 were purified and characterized. The enzymes were most active at neutral pH and temperatures up to 45 °C. All enzymes released ferulic acid and p-coumaric acid from a soluble corn fibre fraction. Ferulic acid esterases FaeA1 and FaeA2 could also release complex dehydrodiferulic acids and dehydrotriferulic acids from corn fibre oligomers, but released only 20% of all ferulic acid present in sugar beet pectin oligomers. Ferulic acid esterase FaeB2 released almost no complex ferulic acid oligomers from corn fibre oligomers, but 60% of all ferulic acid from sugar beet pectin oligomers. The ferulic acid esterases were classified based on both, sequence similarity and their activities toward synthetic substrates. The type A ferulic acid esterases FaeA1 and FaeA2 are the first members of the phylogenetic subfamily 5 to be biochemically characterized. Type B ferulic acid esterase FaeB2 is a member of subfamily 6.
Original languageEnglish
Pages (from-to)77-85
JournalEnzyme and Microbial Technology
Volume50
Issue number1
DOIs
Publication statusPublished - 2012

Keywords

  • sugar-beet pulp
  • plant-cell walls
  • aspergillus-niger
  • maize bran
  • feruloylated oligosaccharides
  • structural-characterization
  • wheat bran
  • degradation
  • classification
  • arabinoxylans

Fingerprint Dive into the research topics of 'The ferulic acid esterases of Chrysosporium lucknowense C1: Purification, characterization and their potential application in biorefinery'. Together they form a unique fingerprint.

Cite this