GP64 and F proteins were previously identified as the principle functional envelope fusion proteins in the Baculoviridae. The F-like proteins in group I NPVs, which use GP64 as a functional envelope fusion protein, are thought to be remnants of F in group I NPVs and lack a furin cleavage site. The F-like proteins were demonstrated to be a pathogenicity factor in vivo, but to be irrelevant for budded virus production in cell culture. However, in the current study we analyzed the function of the F-like protein Ac23 of Autographa californica MNPV in the absence of Ac-GP64 and found that the presence of Ac23 significantly enhanced infectious BV production of gp64 null-AcMNPV pseudotyped with Spodoptera exigua MNPV F protein. Quantitative PCR further revealed the absence of Ac23 leads to a lower production of infectious progeny virions as compared to control viruses. These findings provide evidence that the F-like protein not only enhances viral infectivity in vivo, but also in vitro. The role of the F-like protein as an auxiliary factor in BV maturation in group I NPVs will be further discussed.
|Title of host publication||Abstract Book of the 41st Annual Meeting of the Society for Invertebrate Pathology and 9th International Conference on Bacillus thuringiensis, Warwick, United Kingdom, 3-7 August 2008|
|Place of Publication||Warwick|
|Publication status||Published - 2008|
|Event||41st Annual Meeting of the Society for Invertebrate Pathology and the 9th International Conference on Bacillus thuringiensis - |
Duration: 3 Aug 2008 → 7 Aug 2008
|Conference||41st Annual Meeting of the Society for Invertebrate Pathology and the 9th International Conference on Bacillus thuringiensis|
|Period||3/08/08 → 7/08/08|