The enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment

H.J. Boot; A.J.W. Hoekman; A.L.J. Gielkens

doi:10.1007/s00705-004-0405-9

The enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment

H.J. Boot, A.J.W. Hoekman, A.L.J. Gielkens

Research output: Contribution to journal › Article › Academic › peer-review

49 Citations (Scopus)

Abstract

There is a remarkable difference in virulence of infectious bursal disease virus (IBDV) strains ranging from sub-clinical infections for serotype 2 and cell culture adapted serotype 1 strains, to 100% mortality for very virulent serotype 1 strains in young SPF chickens. It is known that cell culture adaptation related attenuation is determined by distinct mutations in the hypervariable region of the VP2 outer capsid protein, encoded on the A-segment. Amino acid mutations in the hypervariable VP2 region however, offer no explanation for the difference in virulence of classical and very virulent serotype 1 strains. Here we show by in vitro and in vivo analysis of rescued segment reassorted IBDVs that virulence factors are not only located on the A-segment, but on the RNA Dependent RNA Polymerase (VP1) encoding B-segment as well. Insight into the virulence factors of very virulent IBDV will contribute to the improvement of live IBDV vaccines.

Original language	English
Pages (from-to)	137-144
Journal	Archives of Virology
Volume	150
Issue number	1
DOIs	https://doi.org/10.1007/s00705-004-0405-9
Publication status	Published - 2005

Keywords

type-1 poliovirus
amino-acids
attenuation
vp2
phenotype
adaptation
sequence
strains
vaccine
rescue

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00705-004-0405-9

Cite this

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title = "The enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment",

abstract = "There is a remarkable difference in virulence of infectious bursal disease virus (IBDV) strains ranging from sub-clinical infections for serotype 2 and cell culture adapted serotype 1 strains, to 100% mortality for very virulent serotype 1 strains in young SPF chickens. It is known that cell culture adaptation related attenuation is determined by distinct mutations in the hypervariable region of the VP2 outer capsid protein, encoded on the A-segment. Amino acid mutations in the hypervariable VP2 region however, offer no explanation for the difference in virulence of classical and very virulent serotype 1 strains. Here we show by in vitro and in vivo analysis of rescued segment reassorted IBDVs that virulence factors are not only located on the A-segment, but on the RNA Dependent RNA Polymerase (VP1) encoding B-segment as well. Insight into the virulence factors of very virulent IBDV will contribute to the improvement of live IBDV vaccines.",

keywords = "type-1 poliovirus, amino-acids, attenuation, vp2, phenotype, adaptation, sequence, strains, vaccine, rescue",

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T1 - The enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment

AU - Boot, H.J.

AU - Hoekman, A.J.W.

AU - Gielkens, A.L.J.

N1 - Times Cited: 9

PY - 2005

Y1 - 2005

N2 - There is a remarkable difference in virulence of infectious bursal disease virus (IBDV) strains ranging from sub-clinical infections for serotype 2 and cell culture adapted serotype 1 strains, to 100% mortality for very virulent serotype 1 strains in young SPF chickens. It is known that cell culture adaptation related attenuation is determined by distinct mutations in the hypervariable region of the VP2 outer capsid protein, encoded on the A-segment. Amino acid mutations in the hypervariable VP2 region however, offer no explanation for the difference in virulence of classical and very virulent serotype 1 strains. Here we show by in vitro and in vivo analysis of rescued segment reassorted IBDVs that virulence factors are not only located on the A-segment, but on the RNA Dependent RNA Polymerase (VP1) encoding B-segment as well. Insight into the virulence factors of very virulent IBDV will contribute to the improvement of live IBDV vaccines.

AB - There is a remarkable difference in virulence of infectious bursal disease virus (IBDV) strains ranging from sub-clinical infections for serotype 2 and cell culture adapted serotype 1 strains, to 100% mortality for very virulent serotype 1 strains in young SPF chickens. It is known that cell culture adaptation related attenuation is determined by distinct mutations in the hypervariable region of the VP2 outer capsid protein, encoded on the A-segment. Amino acid mutations in the hypervariable VP2 region however, offer no explanation for the difference in virulence of classical and very virulent serotype 1 strains. Here we show by in vitro and in vivo analysis of rescued segment reassorted IBDVs that virulence factors are not only located on the A-segment, but on the RNA Dependent RNA Polymerase (VP1) encoding B-segment as well. Insight into the virulence factors of very virulent IBDV will contribute to the improvement of live IBDV vaccines.

KW - type-1 poliovirus

KW - amino-acids

KW - attenuation

KW - vp2

KW - phenotype

KW - adaptation

KW - sequence

KW - strains

KW - vaccine

KW - rescue

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DO - 10.1007/s00705-004-0405-9

M3 - Article

SN - 0304-8608

VL - 150

SP - 137

EP - 144

JO - Archives of Virology

JF - Archives of Virology

IS - 1

ER -

The enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment

Abstract

Keywords

UN SDGs

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