The effects of, and interactions between, Cardinium and Wolbachia in the doubly infected spider mite Bryobia sarothamni (Acari: Tetranychidae)

V.I.D. Ros, J.A.J. Breeuwer

Research output: Contribution to journalArticleAcademicpeer-review

62 Citations (Scopus)

Abstract

Many arthropods are infected with vertically transmitted, intracellular bacteria manipulating their host's reproduction. Cytoplasmic incompatibility (CI) is commonly observed and is expressed as a reduction in the number of offspring in crosses between infected males and uninfected females (or females infected with a different bacterial strain). CI is often related to the presence of Wolbachia, but recent findings indicate that a second reproductive parasite, Cardinium, is also capable of inducing CI. Although both Wolbachia and Cardinium occur in arthropods and may infect the same host species, little is known about their interactions. We observed Wolbachia and Cardinium in the sexual spider mite Bryobia sarothamni (Acari: Tetranychidae) and investigated the effects of both bacteria on reproduction. We performed all possible crossing combinations using naturally infected strains, and show that Cardinium induces strong CI, expressed as an almost complete female mortality. B. sarothamni is the third host species in which Cardinium-induced CI is observed, and this study reveals the strongest CI effect found so far. Wolbachia, however, did not induce CI. Even so, CI was not induced by doubly infected males, and neither singly Wolbachia-infected nor doubly infected females could rescue CI induced by Cardinium-infected males. Possibly, this is related to the differences between Cardinium strains infecting singly and doubly infected individuals. We found a cost of infection in single infected individuals, but not in doubly infected individuals. We show that infection frequencies in field populations ranged from completely uninfected to a polymorphic state. In none of the populations infections were fixed
Original languageEnglish
Pages (from-to)413-422
JournalHeredity
Volume102
Issue number4
Publication statusPublished - 2009

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Tetranychidae
Wolbachia
Arthropods
Reproduction
Infection
Bacteria
Population
Parasites
Costs and Cost Analysis
Mortality

Keywords

  • induced cytoplasmic incompatibility
  • tetranychus-urticae
  • drosophila-simulans
  • host genotype
  • nasonia-vitripennis
  • bacterial symbiont
  • haplodiploid mite
  • dna amplification
  • genome sequence
  • fecundity

Cite this

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title = "The effects of, and interactions between, Cardinium and Wolbachia in the doubly infected spider mite Bryobia sarothamni (Acari: Tetranychidae)",
abstract = "Many arthropods are infected with vertically transmitted, intracellular bacteria manipulating their host's reproduction. Cytoplasmic incompatibility (CI) is commonly observed and is expressed as a reduction in the number of offspring in crosses between infected males and uninfected females (or females infected with a different bacterial strain). CI is often related to the presence of Wolbachia, but recent findings indicate that a second reproductive parasite, Cardinium, is also capable of inducing CI. Although both Wolbachia and Cardinium occur in arthropods and may infect the same host species, little is known about their interactions. We observed Wolbachia and Cardinium in the sexual spider mite Bryobia sarothamni (Acari: Tetranychidae) and investigated the effects of both bacteria on reproduction. We performed all possible crossing combinations using naturally infected strains, and show that Cardinium induces strong CI, expressed as an almost complete female mortality. B. sarothamni is the third host species in which Cardinium-induced CI is observed, and this study reveals the strongest CI effect found so far. Wolbachia, however, did not induce CI. Even so, CI was not induced by doubly infected males, and neither singly Wolbachia-infected nor doubly infected females could rescue CI induced by Cardinium-infected males. Possibly, this is related to the differences between Cardinium strains infecting singly and doubly infected individuals. We found a cost of infection in single infected individuals, but not in doubly infected individuals. We show that infection frequencies in field populations ranged from completely uninfected to a polymorphic state. In none of the populations infections were fixed",
keywords = "induced cytoplasmic incompatibility, tetranychus-urticae, drosophila-simulans, host genotype, nasonia-vitripennis, bacterial symbiont, haplodiploid mite, dna amplification, genome sequence, fecundity",
author = "V.I.D. Ros and J.A.J. Breeuwer",
year = "2009",
language = "English",
volume = "102",
pages = "413--422",
journal = "Heredity",
issn = "0018-067X",
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}

The effects of, and interactions between, Cardinium and Wolbachia in the doubly infected spider mite Bryobia sarothamni (Acari: Tetranychidae). / Ros, V.I.D.; Breeuwer, J.A.J.

In: Heredity, Vol. 102, No. 4, 2009, p. 413-422.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The effects of, and interactions between, Cardinium and Wolbachia in the doubly infected spider mite Bryobia sarothamni (Acari: Tetranychidae)

AU - Ros, V.I.D.

AU - Breeuwer, J.A.J.

PY - 2009

Y1 - 2009

N2 - Many arthropods are infected with vertically transmitted, intracellular bacteria manipulating their host's reproduction. Cytoplasmic incompatibility (CI) is commonly observed and is expressed as a reduction in the number of offspring in crosses between infected males and uninfected females (or females infected with a different bacterial strain). CI is often related to the presence of Wolbachia, but recent findings indicate that a second reproductive parasite, Cardinium, is also capable of inducing CI. Although both Wolbachia and Cardinium occur in arthropods and may infect the same host species, little is known about their interactions. We observed Wolbachia and Cardinium in the sexual spider mite Bryobia sarothamni (Acari: Tetranychidae) and investigated the effects of both bacteria on reproduction. We performed all possible crossing combinations using naturally infected strains, and show that Cardinium induces strong CI, expressed as an almost complete female mortality. B. sarothamni is the third host species in which Cardinium-induced CI is observed, and this study reveals the strongest CI effect found so far. Wolbachia, however, did not induce CI. Even so, CI was not induced by doubly infected males, and neither singly Wolbachia-infected nor doubly infected females could rescue CI induced by Cardinium-infected males. Possibly, this is related to the differences between Cardinium strains infecting singly and doubly infected individuals. We found a cost of infection in single infected individuals, but not in doubly infected individuals. We show that infection frequencies in field populations ranged from completely uninfected to a polymorphic state. In none of the populations infections were fixed

AB - Many arthropods are infected with vertically transmitted, intracellular bacteria manipulating their host's reproduction. Cytoplasmic incompatibility (CI) is commonly observed and is expressed as a reduction in the number of offspring in crosses between infected males and uninfected females (or females infected with a different bacterial strain). CI is often related to the presence of Wolbachia, but recent findings indicate that a second reproductive parasite, Cardinium, is also capable of inducing CI. Although both Wolbachia and Cardinium occur in arthropods and may infect the same host species, little is known about their interactions. We observed Wolbachia and Cardinium in the sexual spider mite Bryobia sarothamni (Acari: Tetranychidae) and investigated the effects of both bacteria on reproduction. We performed all possible crossing combinations using naturally infected strains, and show that Cardinium induces strong CI, expressed as an almost complete female mortality. B. sarothamni is the third host species in which Cardinium-induced CI is observed, and this study reveals the strongest CI effect found so far. Wolbachia, however, did not induce CI. Even so, CI was not induced by doubly infected males, and neither singly Wolbachia-infected nor doubly infected females could rescue CI induced by Cardinium-infected males. Possibly, this is related to the differences between Cardinium strains infecting singly and doubly infected individuals. We found a cost of infection in single infected individuals, but not in doubly infected individuals. We show that infection frequencies in field populations ranged from completely uninfected to a polymorphic state. In none of the populations infections were fixed

KW - induced cytoplasmic incompatibility

KW - tetranychus-urticae

KW - drosophila-simulans

KW - host genotype

KW - nasonia-vitripennis

KW - bacterial symbiont

KW - haplodiploid mite

KW - dna amplification

KW - genome sequence

KW - fecundity

M3 - Article

VL - 102

SP - 413

EP - 422

JO - Heredity

JF - Heredity

SN - 0018-067X

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ER -