The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life

Klaudyna Borewicz*, Maria Suarez-Diez, Christine Hechler, Roseriet Beijers, Carolina de Weerth, Ilja Arts, John Penders, Carel Thijs, Arjen Nauta, Cordula Lindner, Ellen Van Leusen, Elaine E. Vaughan, Hauke Smidt

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today’s infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2–12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002–2003, which contained no added prebiotics. We showed that today’s formulas supplemented with either scGOS (0.24–0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.

Original languageEnglish
Article number2434
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 21 Feb 2019

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Prebiotics
Infant Formula
Microbiota
Bifidobacterium
Human Milk
rRNA Genes
Netherlands
Gastrointestinal Tract
Polymerase Chain Reaction

Cite this

Borewicz, Klaudyna ; Suarez-Diez, Maria ; Hechler, Christine ; Beijers, Roseriet ; de Weerth, Carolina ; Arts, Ilja ; Penders, John ; Thijs, Carel ; Nauta, Arjen ; Lindner, Cordula ; Van Leusen, Ellen ; Vaughan, Elaine E. ; Smidt, Hauke. / The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today’s infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2–12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002–2003, which contained no added prebiotics. We showed that today’s formulas supplemented with either scGOS (0.24–0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.",
author = "Klaudyna Borewicz and Maria Suarez-Diez and Christine Hechler and Roseriet Beijers and {de Weerth}, Carolina and Ilja Arts and John Penders and Carel Thijs and Arjen Nauta and Cordula Lindner and {Van Leusen}, Ellen and Vaughan, {Elaine E.} and Hauke Smidt",
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Borewicz, K, Suarez-Diez, M, Hechler, C, Beijers, R, de Weerth, C, Arts, I, Penders, J, Thijs, C, Nauta, A, Lindner, C, Van Leusen, E, Vaughan, EE & Smidt, H 2019, 'The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life', Scientific Reports, vol. 9, no. 1, 2434. https://doi.org/10.1038/s41598-018-38268-x

The effect of prebiotic fortified infant formulas on microbiota composition and dynamics in early life. / Borewicz, Klaudyna; Suarez-Diez, Maria; Hechler, Christine; Beijers, Roseriet; de Weerth, Carolina; Arts, Ilja; Penders, John; Thijs, Carel; Nauta, Arjen; Lindner, Cordula; Van Leusen, Ellen; Vaughan, Elaine E.; Smidt, Hauke.

In: Scientific Reports, Vol. 9, No. 1, 2434, 21.02.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Borewicz, Klaudyna

AU - Suarez-Diez, Maria

AU - Hechler, Christine

AU - Beijers, Roseriet

AU - de Weerth, Carolina

AU - Arts, Ilja

AU - Penders, John

AU - Thijs, Carel

AU - Nauta, Arjen

AU - Lindner, Cordula

AU - Van Leusen, Ellen

AU - Vaughan, Elaine E.

AU - Smidt, Hauke

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N2 - Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today’s infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2–12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002–2003, which contained no added prebiotics. We showed that today’s formulas supplemented with either scGOS (0.24–0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.

AB - Gastrointestinal (GI) microbiota composition differs between breastfed and formula-fed infants. Today’s infant formulas are often fortified with prebiotics to better mimic properties of human milk with respect to its effect on GI microbiota composition and function. We used Illumina HiSeq sequencing of PCR-amplified 16S rRNA gene fragments to investigate the composition of faecal microbiota in 2–12 week old infants receiving either breastmilk, infant formulas fortified with prebiotics, or mixed feeding. We compared these results with results from infants fed traditional formulas used in the Netherlands in 2002–2003, which contained no added prebiotics. We showed that today’s formulas supplemented with either scGOS (0.24–0.50 g/100 ml) or scGOS and lcFOS (at a 9:1 ratio; total 0.6 g/100 ml) had a strong bifidogenic effect as compared to traditional formulas, and they also resulted in altered patterns of microbial colonisation within the developing infant gastrointestinal tract. We identified three microbial states (or developmental stages) in the first 12 weeks of life, with a gradual transition pattern towards a bifidobacteria dominated state. In infants receiving only fortified formulas, this transition towards the bifidobacteria dominated state was accelerated, whereas in infants receiving mixed feeding the transition was delayed, as compared to exclusively breastfed infants.

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DO - 10.1038/s41598-018-38268-x

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VL - 9

JO - Scientific Reports

JF - Scientific Reports

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