The effect of phytase, xylanase and their combination on growth performance and nutrient utilization in Nile tilapia

Roel M. Maas, Marc C.J. Verdegem, Yueming Dersjant-Li, Johan W. Schrama*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Increasing the inclusion rate of plant ingredients will increase the content of non-starch polysaccharides (NSP) and phytate in the fish feed. Both NSP and phytate are undesired in fish feed due to their anti-nutritional properties. The main objective of the present study was to assess the impact of exogenous enzyme supplementation on growth, body composition, digestibility and the energy, nitrogen and phosphorus balances in Nile tilapia. Four experimental diets were tested in a 2 × 2 factorial arrangement of treatments. The first factor was phytase supplementation at a dose of either 0 or 1000 FTU/kg and the second factor was xylanase supplementation at a dose of 0 or 4000 U/kg. This resulted in a control diet (CON-CON) without enzymes, phytase diet (PHY-CON), xylanase diet (CON-XYL) and a diet with both xylanase and phytase (PHY-XYL). In total 24 tanks (6 replicates/treatment) were used with 30 (mean initial body weight 42 g) fish each. Fish were restrictively (80% of expected satiation) fed the experimental diets for 38 days. Growth was significantly affected by the interaction between phytase and xylanase supplementation (P < 0.05), showing a synergism between both enzymes. Growth at the CON-CON and CON-XYL diets were similar, whereas fish fed the PHY-CON had an improved growth. The effect of phytase supplementation on growth was further enhanced when xyalanse was supplemented (PHY-XYL diet). Phytase significantly improved the digestibility of dry matter, crude protein, carbohydrates, energy, ash, phosphorus and calcium (P < 0.001). Xylanase enhanced the digestibility of dry matter, crude protein, carbohydrates and energy significantly (P < 0.05). In contrast to growth, there was no significant synergetic effect of the combination of phytase and xylanase on the digestibility (P > 0.05). The significant synergetic effect of the combination of phytase and xylanase on growth was not reflected on the digestibility (P > 0.05). The nitrogen balance showed that the synergism on growth was predominantly due the significant synergistic effect of phytase and xylanase on the protein retention (P = 0.005). Both xylanase and phytase showed to be an effective tool to improve the nutrient availability and growth in Nile tilapia. Fish fed the diet supplemented with both phytase and xylanase had a significantly higher growth than all other treatments.
Original languageEnglish
Pages (from-to)7-14
JournalAquaculture
Volume487
DOIs
Publication statusPublished - 25 Feb 2018

Fingerprint

nutrient utilization
phytases
xylanases
Oreochromis niloticus
growth performance
diet
nutrient
fish
fish feeds
digestibility
polysaccharide
phytic acid
experimental diets
enzyme
polysaccharides
synergism
nitrogen
effect
nutrient availability
nitrogen balance

Keywords

  • Fish
  • Nitrogen balance
  • Phytase
  • Synergy
  • Xylanase

Cite this

@article{c0f26a4205444f9281857f88ad1e4b87,
title = "The effect of phytase, xylanase and their combination on growth performance and nutrient utilization in Nile tilapia",
abstract = "Increasing the inclusion rate of plant ingredients will increase the content of non-starch polysaccharides (NSP) and phytate in the fish feed. Both NSP and phytate are undesired in fish feed due to their anti-nutritional properties. The main objective of the present study was to assess the impact of exogenous enzyme supplementation on growth, body composition, digestibility and the energy, nitrogen and phosphorus balances in Nile tilapia. Four experimental diets were tested in a 2 × 2 factorial arrangement of treatments. The first factor was phytase supplementation at a dose of either 0 or 1000 FTU/kg and the second factor was xylanase supplementation at a dose of 0 or 4000 U/kg. This resulted in a control diet (CON-CON) without enzymes, phytase diet (PHY-CON), xylanase diet (CON-XYL) and a diet with both xylanase and phytase (PHY-XYL). In total 24 tanks (6 replicates/treatment) were used with 30 (mean initial body weight 42 g) fish each. Fish were restrictively (80{\%} of expected satiation) fed the experimental diets for 38 days. Growth was significantly affected by the interaction between phytase and xylanase supplementation (P < 0.05), showing a synergism between both enzymes. Growth at the CON-CON and CON-XYL diets were similar, whereas fish fed the PHY-CON had an improved growth. The effect of phytase supplementation on growth was further enhanced when xyalanse was supplemented (PHY-XYL diet). Phytase significantly improved the digestibility of dry matter, crude protein, carbohydrates, energy, ash, phosphorus and calcium (P < 0.001). Xylanase enhanced the digestibility of dry matter, crude protein, carbohydrates and energy significantly (P < 0.05). In contrast to growth, there was no significant synergetic effect of the combination of phytase and xylanase on the digestibility (P > 0.05). The significant synergetic effect of the combination of phytase and xylanase on growth was not reflected on the digestibility (P > 0.05). The nitrogen balance showed that the synergism on growth was predominantly due the significant synergistic effect of phytase and xylanase on the protein retention (P = 0.005). Both xylanase and phytase showed to be an effective tool to improve the nutrient availability and growth in Nile tilapia. Fish fed the diet supplemented with both phytase and xylanase had a significantly higher growth than all other treatments.",
keywords = "Fish, Nitrogen balance, Phytase, Synergy, Xylanase",
author = "Maas, {Roel M.} and Verdegem, {Marc C.J.} and Yueming Dersjant-Li and Schrama, {Johan W.}",
year = "2018",
month = "2",
day = "25",
doi = "10.1016/j.aquaculture.2017.12.040",
language = "English",
volume = "487",
pages = "7--14",
journal = "Aquaculture",
issn = "0044-8486",
publisher = "Elsevier",

}

The effect of phytase, xylanase and their combination on growth performance and nutrient utilization in Nile tilapia. / Maas, Roel M.; Verdegem, Marc C.J.; Dersjant-Li, Yueming; Schrama, Johan W.

In: Aquaculture, Vol. 487, 25.02.2018, p. 7-14.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The effect of phytase, xylanase and their combination on growth performance and nutrient utilization in Nile tilapia

AU - Maas, Roel M.

AU - Verdegem, Marc C.J.

AU - Dersjant-Li, Yueming

AU - Schrama, Johan W.

PY - 2018/2/25

Y1 - 2018/2/25

N2 - Increasing the inclusion rate of plant ingredients will increase the content of non-starch polysaccharides (NSP) and phytate in the fish feed. Both NSP and phytate are undesired in fish feed due to their anti-nutritional properties. The main objective of the present study was to assess the impact of exogenous enzyme supplementation on growth, body composition, digestibility and the energy, nitrogen and phosphorus balances in Nile tilapia. Four experimental diets were tested in a 2 × 2 factorial arrangement of treatments. The first factor was phytase supplementation at a dose of either 0 or 1000 FTU/kg and the second factor was xylanase supplementation at a dose of 0 or 4000 U/kg. This resulted in a control diet (CON-CON) without enzymes, phytase diet (PHY-CON), xylanase diet (CON-XYL) and a diet with both xylanase and phytase (PHY-XYL). In total 24 tanks (6 replicates/treatment) were used with 30 (mean initial body weight 42 g) fish each. Fish were restrictively (80% of expected satiation) fed the experimental diets for 38 days. Growth was significantly affected by the interaction between phytase and xylanase supplementation (P < 0.05), showing a synergism between both enzymes. Growth at the CON-CON and CON-XYL diets were similar, whereas fish fed the PHY-CON had an improved growth. The effect of phytase supplementation on growth was further enhanced when xyalanse was supplemented (PHY-XYL diet). Phytase significantly improved the digestibility of dry matter, crude protein, carbohydrates, energy, ash, phosphorus and calcium (P < 0.001). Xylanase enhanced the digestibility of dry matter, crude protein, carbohydrates and energy significantly (P < 0.05). In contrast to growth, there was no significant synergetic effect of the combination of phytase and xylanase on the digestibility (P > 0.05). The significant synergetic effect of the combination of phytase and xylanase on growth was not reflected on the digestibility (P > 0.05). The nitrogen balance showed that the synergism on growth was predominantly due the significant synergistic effect of phytase and xylanase on the protein retention (P = 0.005). Both xylanase and phytase showed to be an effective tool to improve the nutrient availability and growth in Nile tilapia. Fish fed the diet supplemented with both phytase and xylanase had a significantly higher growth than all other treatments.

AB - Increasing the inclusion rate of plant ingredients will increase the content of non-starch polysaccharides (NSP) and phytate in the fish feed. Both NSP and phytate are undesired in fish feed due to their anti-nutritional properties. The main objective of the present study was to assess the impact of exogenous enzyme supplementation on growth, body composition, digestibility and the energy, nitrogen and phosphorus balances in Nile tilapia. Four experimental diets were tested in a 2 × 2 factorial arrangement of treatments. The first factor was phytase supplementation at a dose of either 0 or 1000 FTU/kg and the second factor was xylanase supplementation at a dose of 0 or 4000 U/kg. This resulted in a control diet (CON-CON) without enzymes, phytase diet (PHY-CON), xylanase diet (CON-XYL) and a diet with both xylanase and phytase (PHY-XYL). In total 24 tanks (6 replicates/treatment) were used with 30 (mean initial body weight 42 g) fish each. Fish were restrictively (80% of expected satiation) fed the experimental diets for 38 days. Growth was significantly affected by the interaction between phytase and xylanase supplementation (P < 0.05), showing a synergism between both enzymes. Growth at the CON-CON and CON-XYL diets were similar, whereas fish fed the PHY-CON had an improved growth. The effect of phytase supplementation on growth was further enhanced when xyalanse was supplemented (PHY-XYL diet). Phytase significantly improved the digestibility of dry matter, crude protein, carbohydrates, energy, ash, phosphorus and calcium (P < 0.001). Xylanase enhanced the digestibility of dry matter, crude protein, carbohydrates and energy significantly (P < 0.05). In contrast to growth, there was no significant synergetic effect of the combination of phytase and xylanase on the digestibility (P > 0.05). The significant synergetic effect of the combination of phytase and xylanase on growth was not reflected on the digestibility (P > 0.05). The nitrogen balance showed that the synergism on growth was predominantly due the significant synergistic effect of phytase and xylanase on the protein retention (P = 0.005). Both xylanase and phytase showed to be an effective tool to improve the nutrient availability and growth in Nile tilapia. Fish fed the diet supplemented with both phytase and xylanase had a significantly higher growth than all other treatments.

KW - Fish

KW - Nitrogen balance

KW - Phytase

KW - Synergy

KW - Xylanase

U2 - 10.1016/j.aquaculture.2017.12.040

DO - 10.1016/j.aquaculture.2017.12.040

M3 - Article

VL - 487

SP - 7

EP - 14

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -