The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7

Maria Juliana Romero-Guzmán, Vasileios Petris, Simone De Chirico, Vincenzo di Bari, David Gray, Remko M. Boom, Constantinos V. Nikiforidis*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Oleosomes are storage vehicles of TAGs in plant seeds. They are protected with a phospholipid-protein monolayer and extracted with alkaline aqueous media; however, pH adjustment intensifies the extraction process. Therefore, the aim of this work was to investigate the extraction mechanism of rapeseed oleosomes at pH 7 and at the presence of monovalent and divalent cations (Na+, K+, Mg2+ , and Ca+2). The oleosome yield at pH 9.5 was 64 wt%, while the yield at pH 7 with H2O was just 43 wt.%. The presence of cations at pH 7, significantly enhanced the yield, with K+ giving the highest yield (64 wt.%). The cations affected the oleosome interface and their interactions. The presence of monovalent cations resulted in aggregation and minor coalescence, while divalent cations resulted in extensive coalescence. These results help to understand the interactions of oleosomes in their native matrix and design simple extraction processes at neutral conditions.

Original languageEnglish
Article number125578
JournalFood Chemistry
Volume306
DOIs
Publication statusPublished - 15 Feb 2020

Fingerprint

lipid bodies
rapeseed
Cations
Monovalent Cations
cations
Divalent Cations
Coalescence
calcium
Seed
Monolayers
Phospholipids
Agglomeration
Brassica rapa
Spermatophytina
Seeds
phospholipids
rapeseed oil
Lipid Droplets
Proteins
proteins

Keywords

  • Extraction
  • Natural emulsion
  • Oil bodies
  • Oleosomes
  • Rapeseed

Cite this

Romero-Guzmán, Maria Juliana ; Petris, Vasileios ; De Chirico, Simone ; di Bari, Vincenzo ; Gray, David ; Boom, Remko M. ; Nikiforidis, Constantinos V. / The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7. In: Food Chemistry. 2020 ; Vol. 306.
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title = "The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7",
abstract = "Oleosomes are storage vehicles of TAGs in plant seeds. They are protected with a phospholipid-protein monolayer and extracted with alkaline aqueous media; however, pH adjustment intensifies the extraction process. Therefore, the aim of this work was to investigate the extraction mechanism of rapeseed oleosomes at pH 7 and at the presence of monovalent and divalent cations (Na+, K+, Mg2+ , and Ca+2). The oleosome yield at pH 9.5 was 64 wt{\%}, while the yield at pH 7 with H2O was just 43 wt.{\%}. The presence of cations at pH 7, significantly enhanced the yield, with K+ giving the highest yield (64 wt.{\%}). The cations affected the oleosome interface and their interactions. The presence of monovalent cations resulted in aggregation and minor coalescence, while divalent cations resulted in extensive coalescence. These results help to understand the interactions of oleosomes in their native matrix and design simple extraction processes at neutral conditions.",
keywords = "Extraction, Natural emulsion, Oil bodies, Oleosomes, Rapeseed",
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The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7. / Romero-Guzmán, Maria Juliana; Petris, Vasileios; De Chirico, Simone; di Bari, Vincenzo; Gray, David; Boom, Remko M.; Nikiforidis, Constantinos V.

In: Food Chemistry, Vol. 306, 125578, 15.02.2020.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7

AU - Romero-Guzmán, Maria Juliana

AU - Petris, Vasileios

AU - De Chirico, Simone

AU - di Bari, Vincenzo

AU - Gray, David

AU - Boom, Remko M.

AU - Nikiforidis, Constantinos V.

PY - 2020/2/15

Y1 - 2020/2/15

N2 - Oleosomes are storage vehicles of TAGs in plant seeds. They are protected with a phospholipid-protein monolayer and extracted with alkaline aqueous media; however, pH adjustment intensifies the extraction process. Therefore, the aim of this work was to investigate the extraction mechanism of rapeseed oleosomes at pH 7 and at the presence of monovalent and divalent cations (Na+, K+, Mg2+ , and Ca+2). The oleosome yield at pH 9.5 was 64 wt%, while the yield at pH 7 with H2O was just 43 wt.%. The presence of cations at pH 7, significantly enhanced the yield, with K+ giving the highest yield (64 wt.%). The cations affected the oleosome interface and their interactions. The presence of monovalent cations resulted in aggregation and minor coalescence, while divalent cations resulted in extensive coalescence. These results help to understand the interactions of oleosomes in their native matrix and design simple extraction processes at neutral conditions.

AB - Oleosomes are storage vehicles of TAGs in plant seeds. They are protected with a phospholipid-protein monolayer and extracted with alkaline aqueous media; however, pH adjustment intensifies the extraction process. Therefore, the aim of this work was to investigate the extraction mechanism of rapeseed oleosomes at pH 7 and at the presence of monovalent and divalent cations (Na+, K+, Mg2+ , and Ca+2). The oleosome yield at pH 9.5 was 64 wt%, while the yield at pH 7 with H2O was just 43 wt.%. The presence of cations at pH 7, significantly enhanced the yield, with K+ giving the highest yield (64 wt.%). The cations affected the oleosome interface and their interactions. The presence of monovalent cations resulted in aggregation and minor coalescence, while divalent cations resulted in extensive coalescence. These results help to understand the interactions of oleosomes in their native matrix and design simple extraction processes at neutral conditions.

KW - Extraction

KW - Natural emulsion

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KW - Oleosomes

KW - Rapeseed

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