The effect of medium composition on ovary-slice culture and ovule culture in intraspecific Tulipa gesneriana crosses

M.G.M. van Creij, D.M.F.J. Kerckhoffs, S.M. de Bruijn, J.M. van Tuyl, D. Vreugdenhil

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15 Citations (Scopus)


The effect of several media components on the germination percentage of ovules in intraspecific T. gesneriana L. crosses was studied by using two embryo rescue techniques, viz. ovary-slice culture followed by ovule culture and direct ovule culture. The addition of 9% sucrose to medium for ovary-slice culture, started at 3 or at 5 weeks after pollination (WAP), significantly improved the germination percentage as compared to 5% sucrose. The germination percentage did not differ between both sucrose concentrations (3% and 5%) used in ovule culture started 4 weeks later with ovules excised from the ovary-slices (at 9 WAP). Similar germination percentages were obtained with media containing the full or half of the concentrations of micronutrients and macronutrients of the MS-medium during ovary-slice culture and ovule culture. For direct ovule culture, started at 4, at 6, and at 8 WAP, the germination percentages did not differ between ovules cultured on media with 3%, 6% or 9% sucrose. The addition of the cytokinin BAP (0.01 or 0.1 mg l-1) had no effect on the germination percentage. The use of liquid-shaken culture resulted in germination percentages which were similar to those on agar-solidified media. Analysis of the carbohydrate concentration of the media revealed that, in both media for ovary-slice culture and for ovule culture, ultimately all sucrose is converted into glucose and fructose. The total concentration of carbohydrates decreased with 19%–48% in the media for ovary-slice culture, whereas the total concentration of carbohydrates did not decrease remarkably in media for ovule culture.
Original languageEnglish
Pages (from-to)61-67
JournalPlant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
Publication statusPublished - 2000


  • Carbohydrates
  • Embryo Rescue
  • Media Composition
  • Tulip
  • Tulipa gesneriana


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