The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation

J.B. Kim; M.J. de Jeu; C.J.J.M. Raemakers; E. Jacobsen; R.G.F. Visser

The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation

J.B. Kim, M.J. de Jeu, C.J.J.M. Raemakers, E. Jacobsen, R.G.F. Visser

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

Abstract

This is the first successful report of Agrobacterium-mediated transformation in Alstroemeria by infection of FEC (Friable Embryogenic Callus) lines and leaves with axil tissues. Of the transformation methods, particle bombardment and Agrobacterium-mediated transformation have been widely used to transfer foreign DNA into the plant genome. Especially, Agrobacterium tumefaciens efficiently infects most plants. Most monocotyledonous plants, including Alstroemeria, are recalcitrant to A. tumefaciens infection. Therefore, it is essential to develop an efficient, reliable protocol for Agrobacterium-mediated transformation in Alstroemeria. In order to achieve this aim, different hygromycin concentrations in selection medium were tested and compared in FEC explants and leaves with axil tissues using Agrobacterium strain LBA4404 (pTOK233). As a result, 20 mg/l hygromycin was a proper concentration to select for both FEC lines and leaves with axil tissues. Using this concentration, transformed lines were obtained. Moreover, FEC lines showed at least 2.5 times higher rate in the survival rate, and 5 times higher rate in transient GUS gene expression than those of leaves with axil tissues. The protocol, seems to be a promising method for the transformation of the monocot Alstroemeria with genes of interest in the near future.

Original language	English
Title of host publication	Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001
Editors	J. Van Huylenbroeck, E. Van Bockstaele
Pages	97-104
Publication status	Published - 2002
Event	XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals - Melle, Ghent, Belgium Duration: 3 Jul 2001 → 6 Jul 2001

Publication series

Name	Acta Horticulturae
Number	572
ISSN (Print)	0567-7572

Conference/symposium

Conference/symposium	XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals
City	Melle, Ghent, Belgium
Period	3/07/01 → 6/07/01

Keywords

Friable embryogenic callus
GUS
Leaves with axils
Monocot
Organogenic tissue
Somatic embryo

Access to Document

https://edepot.wur.nl/18477

Cite this

Kim, J. B., de Jeu, M. J., Raemakers, C. J. J. M., Jacobsen, E., & Visser, R. G. F. (2002). The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation. In J. Van Huylenbroeck, & E. Van Bockstaele (Eds.), Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001 (pp. 97-104). (Acta Horticulturae; No. 572). https://edepot.wur.nl/18477

Kim, J.B. ; de Jeu, M.J. ; Raemakers, C.J.J.M. et al. / The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation. Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001. editor / J. Van Huylenbroeck ; E. Van Bockstaele. 2002. pp. 97-104 (Acta Horticulturae; 572).

@inproceedings{4ce2846c69cf4097bdf1e947b1d356f1,

title = "The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation",

abstract = "This is the first successful report of Agrobacterium-mediated transformation in Alstroemeria by infection of FEC (Friable Embryogenic Callus) lines and leaves with axil tissues. Of the transformation methods, particle bombardment and Agrobacterium-mediated transformation have been widely used to transfer foreign DNA into the plant genome. Especially, Agrobacterium tumefaciens efficiently infects most plants. Most monocotyledonous plants, including Alstroemeria, are recalcitrant to A. tumefaciens infection. Therefore, it is essential to develop an efficient, reliable protocol for Agrobacterium-mediated transformation in Alstroemeria. In order to achieve this aim, different hygromycin concentrations in selection medium were tested and compared in FEC explants and leaves with axil tissues using Agrobacterium strain LBA4404 (pTOK233). As a result, 20 mg/l hygromycin was a proper concentration to select for both FEC lines and leaves with axil tissues. Using this concentration, transformed lines were obtained. Moreover, FEC lines showed at least 2.5 times higher rate in the survival rate, and 5 times higher rate in transient GUS gene expression than those of leaves with axil tissues. The protocol, seems to be a promising method for the transformation of the monocot Alstroemeria with genes of interest in the near future.",

keywords = "Friable embryogenic callus, GUS, Leaves with axils, Monocot, Organogenic tissue, Somatic embryo",

author = "J.B. Kim and {de Jeu}, M.J. and C.J.J.M. Raemakers and E. Jacobsen and R.G.F. Visser",

year = "2002",

language = "English",

isbn = "9789066058255",

series = "Acta Horticulturae",

number = "572",

pages = "97--104",

editor = "{Van Huylenbroeck}, J. and {Van Bockstaele}, E.",

booktitle = "Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001",

note = "XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals ; Conference date: 03-07-2001 Through 06-07-2001",

}

Kim, JB, de Jeu, MJ, Raemakers, CJJM, Jacobsen, E & Visser, RGF 2002, The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation. in J Van Huylenbroeck & E Van Bockstaele (eds), Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001. Acta Horticulturae, no. 572, pp. 97-104, XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals, Melle, Ghent, Belgium, 3/07/01. <https://edepot.wur.nl/18477>

The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation. / Kim, J.B.; de Jeu, M.J.; Raemakers, C.J.J.M. et al.
Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001. ed. / J. Van Huylenbroeck; E. Van Bockstaele. 2002. p. 97-104 (Acta Horticulturae; No. 572).

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

TY - GEN

T1 - The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation

AU - Kim, J.B.

AU - de Jeu, M.J.

AU - Raemakers, C.J.J.M.

AU - Jacobsen, E.

AU - Visser, R.G.F.

PY - 2002

Y1 - 2002

N2 - This is the first successful report of Agrobacterium-mediated transformation in Alstroemeria by infection of FEC (Friable Embryogenic Callus) lines and leaves with axil tissues. Of the transformation methods, particle bombardment and Agrobacterium-mediated transformation have been widely used to transfer foreign DNA into the plant genome. Especially, Agrobacterium tumefaciens efficiently infects most plants. Most monocotyledonous plants, including Alstroemeria, are recalcitrant to A. tumefaciens infection. Therefore, it is essential to develop an efficient, reliable protocol for Agrobacterium-mediated transformation in Alstroemeria. In order to achieve this aim, different hygromycin concentrations in selection medium were tested and compared in FEC explants and leaves with axil tissues using Agrobacterium strain LBA4404 (pTOK233). As a result, 20 mg/l hygromycin was a proper concentration to select for both FEC lines and leaves with axil tissues. Using this concentration, transformed lines were obtained. Moreover, FEC lines showed at least 2.5 times higher rate in the survival rate, and 5 times higher rate in transient GUS gene expression than those of leaves with axil tissues. The protocol, seems to be a promising method for the transformation of the monocot Alstroemeria with genes of interest in the near future.

AB - This is the first successful report of Agrobacterium-mediated transformation in Alstroemeria by infection of FEC (Friable Embryogenic Callus) lines and leaves with axil tissues. Of the transformation methods, particle bombardment and Agrobacterium-mediated transformation have been widely used to transfer foreign DNA into the plant genome. Especially, Agrobacterium tumefaciens efficiently infects most plants. Most monocotyledonous plants, including Alstroemeria, are recalcitrant to A. tumefaciens infection. Therefore, it is essential to develop an efficient, reliable protocol for Agrobacterium-mediated transformation in Alstroemeria. In order to achieve this aim, different hygromycin concentrations in selection medium were tested and compared in FEC explants and leaves with axil tissues using Agrobacterium strain LBA4404 (pTOK233). As a result, 20 mg/l hygromycin was a proper concentration to select for both FEC lines and leaves with axil tissues. Using this concentration, transformed lines were obtained. Moreover, FEC lines showed at least 2.5 times higher rate in the survival rate, and 5 times higher rate in transient GUS gene expression than those of leaves with axil tissues. The protocol, seems to be a promising method for the transformation of the monocot Alstroemeria with genes of interest in the near future.

KW - Friable embryogenic callus

KW - GUS

KW - Leaves with axils

KW - Monocot

KW - Organogenic tissue

KW - Somatic embryo

M3 - Conference paper

SN - 9789066058255

T3 - Acta Horticulturae

SP - 97

EP - 104

BT - Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001

A2 - Van Huylenbroeck, J.

A2 - Van Bockstaele, E.

T2 - XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals

Y2 - 3 July 2001 through 6 July 2001

ER -

Kim JB, de Jeu MJ, Raemakers CJJM, Jacobsen E, Visser RGF. The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation. In Van Huylenbroeck J, Van Bockstaele E, editors, Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001. 2002. p. 97-104. (Acta Horticulturae; 572).

The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation

Abstract

Publication series

Conference/symposium

Keywords

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