The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation

J.B. Kim, M.J. de Jeu, C.J.J.M. Raemakers, E. Jacobsen, R.G.F. Visser

Research output: Chapter in Book/Report/Conference proceedingConference paper

1 Citation (Scopus)

Abstract

This is the first successful report of Agrobacterium-mediated transformation in Alstroemeria by infection of FEC (Friable Embryogenic Callus) lines and leaves with axil tissues. Of the transformation methods, particle bombardment and Agrobacterium-mediated transformation have been widely used to transfer foreign DNA into the plant genome. Especially, Agrobacterium tumefaciens efficiently infects most plants. Most monocotyledonous plants, including Alstroemeria, are recalcitrant to A. tumefaciens infection. Therefore, it is essential to develop an efficient, reliable protocol for Agrobacterium-mediated transformation in Alstroemeria. In order to achieve this aim, different hygromycin concentrations in selection medium were tested and compared in FEC explants and leaves with axil tissues using Agrobacterium strain LBA4404 (pTOK233). As a result, 20 mg/l hygromycin was a proper concentration to select for both FEC lines and leaves with axil tissues. Using this concentration, transformed lines were obtained. Moreover, FEC lines showed at least 2.5 times higher rate in the survival rate, and 5 times higher rate in transient GUS gene expression than those of leaves with axil tissues. The protocol, seems to be a promising method for the transformation of the monocot Alstroemeria with genes of interest in the near future.
Original languageEnglish
Title of host publicationStrategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001
EditorsJ. Van Huylenbroeck, E. Van Bockstaele
Pages97-104
Publication statusPublished - 2002
EventXX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals - Melle, Ghent, Belgium
Duration: 3 Jul 20016 Jul 2001

Publication series

NameActa Horticulturae
Number572
ISSN (Print)0567-7572

Conference

ConferenceXX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals
CityMelle, Ghent, Belgium
Period3/07/016/07/01

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Kim, J. B., de Jeu, M. J., Raemakers, C. J. J. M., Jacobsen, E., & Visser, R. G. F. (2002). The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation. In J. Van Huylenbroeck, & E. Van Bockstaele (Eds.), Strategies for new ornamentals : proceedings of the twentieth international EUCARPIA symposium Section Ornamentals Melle, Belgium, 3 - 6 July, 2001 (pp. 97-104). (Acta Horticulturae; No. 572).