The effect of aluminium on phosphate uptake by three isolated ectomycorrhizal fungi

R.H. Jongbloed*, M.W.A. Tosserams, G.W.F.H. Borst-Pauwels

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Three ectomycorrhizal fungi, Laccaria bicolor, Lactarius hepaticus and Lactarius rufus, were grown in liquid medium for 2 weeks and used in short-term phosphate (P) uptake studies. At phosphate concentrations ranging from 1 to 100 μM the fungi exhibited a single affinity phase. The kinetic parameters for P uptake differed between species with Km varying from 1.5 to 13.1 μM. Vmax also depended on the buffer that was applied. P uptake by the fungi was severely reduced by aluminium (Al). This could be ascribed mainly to the complexing of phosphate by Al3+. Correcting for this decrease in phosphate concentration, the uptake by both Lactarius hepaticus and Laccaria bicolor was only slightly inhibited by Al, while P uptake by Lactarius rufus was even stimulated. The latter may be attributed to a reduction in surface potential caused by the trivalent Al cation, which in turn increases the concentration of the negatively charged phosphate ion in the near-membrane region.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalPlant and Soil
Volume140
Issue number2
DOIs
Publication statusPublished - Feb 1992
Externally publishedYes

Keywords

  • aluminium
  • ectomycorrhizal fungi
  • kinetics
  • Laccaria bicolor
  • Lactarius hepaticus
  • Lactarius rufus
  • liquid culture
  • P uptake

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