The effect of a single bout of exercise on vitamin B2 status is not different between high-and low-fit females

Joëlle J.E. Janssen, Bart Lagerwaard, Silvie Timmers, Vincent C.J. de Boer, Jaap Keijer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)


High-fitness individuals have been suggested to be at risk of a poor vitamin B2 (riboflavin) status due to a potentially higher vitamin B2 demand, as measured by the erythrocyte glutathione reductase (EGR) activation coefficient (EGRAC). Longer-term exercise interventions have been shown to result in a lower vitamin B2 status, but studies are contradictory. Short-term exercise effects potentially contribute to discrepancies between studies but have only been tested in limited study populations. This study investigated if vitamin B2 status, measured by EGRAC, is affected by a single exercise bout in females who differ in fitness levels, and that represents long-term physical activity. At baseline and overnight after a 60-min cycling bout at 70% ˙VO2 peak, EGR activity and EGRAC were measured in 31 young female adults, divided into a high-fit (VO2 peak ≥ 47 mL/kg/min, N = 15) and low-fit (VO2 peak ≤ 37 mL/kg/min, N = 16) group. A single exercise bout significantly increased EGR activity in high-fit and low-fit females (Ptime = 0.006). This response was not affected by fitness level (Ptime*group = 0.256). The effect of exercise on EGRAC was not significant (Ptime = 0.079) and not influenced by EGR activity. The exercise response of EGRAC was not significantly different between high-fit and low-fit females (Ptime*group = 0.141). Thus, a single exercise bout increased EGR activity, but did not affect EGRAC, indicating that vitamin B2 status was not affected. The exercise response on EGRAC and EGR did not differ between high-fit and low-fit females.

Original languageEnglish
Article number4097
Issue number11
Publication statusPublished - 16 Nov 2021


  • Erythrocyte glutathione reductase
  • Exercise
  • High-and low-fit females
  • Vitamin B2 status


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