The Do’s and Don’ts of Effectoromics

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

13 Citations (Scopus)


Effectoromics, a high-throughput functional genomics approach that uses effectors to probe plant germplasm to detect R genes, has proven a potent contribution to modern resistance breeding. Advantages of effectoromics are summarized in four aspects: (1) accelerating R gene identification; (2) distinguishing functional redundancy; (3) detecting recognition specificity and (4) assisting in R gene deployment. In this manuscript, we provide suggestions as well as some reminders for applying effectoromics in the breeding process. The two routine functional assays that are widely used, agroinfiltration and agroinfection, are presented. We briefly explain their advantages and disadvantages and provide protocols for applying them in the model system Nicotiana benthamiana as well as in potato (Solanum tuberosum).
Original languageEnglish
Title of host publicationPlant-Pathogen Interactions
Subtitle of host publicationMethods and Protocols
EditorsP. Birch, J.T. Jones, J.I.B. Bos
ISBN (Electronic)9781627039864
ISBN (Print)9781627039857, 9781493963232
Publication statusPublished - 15 Feb 2014

Publication series

NameMethods in Molecular Biology (MIMB)
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


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