Effectoromics, a high-throughput functional genomics approach that uses effectors to probe plant germplasm to detect R genes, has proven a potent contribution to modern resistance breeding. Advantages of effectoromics are summarized in four aspects: (1) accelerating R gene identification; (2) distinguishing functional redundancy; (3) detecting recognition specificity and (4) assisting in R gene deployment. In this manuscript, we provide suggestions as well as some reminders for applying effectoromics in the breeding process. The two routine functional assays that are widely used, agroinfiltration and agroinfection, are presented. We briefly explain their advantages and disadvantages and provide protocols for applying them in the model system Nicotiana benthamiana as well as in potato (Solanum tuberosum).
|Title of host publication||Plant-Pathogen Interactions |
|Subtitle of host publication||Methods and Protocols|
|Editors||P. Birch, J.T. Jones, J.I.B. Bos|
|ISBN (Print)||9781627039857, 9781493963232|
|Publication status||Published - 15 Feb 2014|
|Name||Methods in Molecular Biology (MIMB)|