The diurnal transcriptional landscape of the microalga Tetradesmus obliquus

Benoit M. Carreres, G. Mitsue León-Saiki, Peter J. Schaap, Ilse M. Remmers, Douwe van der Veen, Vitor A.P. Martins dos Santos, René H. Wijffels, Dirk E. Martens*, Maria Suarez-Diez

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Tetradesmus obliquus is a promising oleaginous microalga. We functionally annotated its genome and characterized the transcriptional landscape of T. obliquus adapted to 16:8 h light dark (LD) cycles in turbidostat culture conditions at very high temporal resolution (1 h intervals). Revealing a cycle of cellular events, six distinct expression profiles were obtained, each with transcriptional phenotypes correlating with measurements of biochemical composition. The impact of starch deficiency was studied using the starchless mutant slm1. Significant changes in the transcriptional landscape were observed. Starch deficiency resulted in incapacity to supply energy during the dark period, resulting in a shift of energy demanding processes to an earlier or later time point. Our study provides new perspectives on the role of starch and the adaptation to LD cycles of oleaginous microalgae.

Original languageEnglish
Article number101477
JournalAlgal Research
Volume40
DOIs
Publication statusPublished - 1 Jun 2019

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Keywords

  • Day/night cycles
  • Diurnal transcription changes
  • Microalgae
  • Photosynthetic efficiency
  • Scenedesmus obliquus
  • starchless mutant

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