The diurnal transcriptional landscape of the microalga Tetradesmus obliquus

Benoit M. Carreres; G. Mitsue León-Saiki; Peter J. Schaap; Ilse M. Remmers; Douwe van der Veen; Vitor A.P. Martins dos Santos; René H. Wijffels; Dirk E. Martens; Maria Suarez-Diez

doi:10.1101/425934

The diurnal transcriptional landscape of the microalga Tetradesmus obliquus

Benoit M. Carreres, G. Mitsue León-Saiki, Peter J. Schaap, Ilse M. Remmers, Douwe van der Veen, Vitor A.P. Martins dos Santos, René H. Wijffels, Dirk E. Martens^*, Maria Suarez-Diez

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

Tetradesmus obliquus is a promising oleaginous microalga. We functionally annotated its genome and characterized the transcriptional landscape of T. obliquus adapted to 16:8 h light dark (LD) cycles in turbidostat culture conditions at very high temporal resolution (1 h intervals). Revealing a cycle of cellular events, six distinct expression profiles were obtained, each with transcriptional phenotypes correlating with measurements of biochemical composition. The impact of starch deficiency was studied using the starchless mutant slm1. Significant changes in the transcriptional landscape were observed. Starch deficiency resulted in incapacity to supply energy during the dark period, resulting in a shift of energy demanding processes to an earlier or later time point. Our study provides new perspectives on the role of starch and the adaptation to LD cycles of oleaginous microalgae.

Original language	English
Article number	101477
Journal	Algal Research
Volume	40
DOIs	https://doi.org/10.1101/425934 https://doi.org/10.1016/j.algal.2019.101477
Publication status	Published - 1 Jun 2019

Keywords

Day/night cycles
Diurnal transcription changes
Microalgae
Photosynthetic efficiency
Scenedesmus obliquus
starchless mutant

Access to Document

10.1101/425934Licence: CC BY-ND
10.1016/j.algal.2019.101477

https://edepot.wur.nl/476174Licence: CC BY-NC-ND

Transcriptional and physiological diurnal responses of Tetradesmus obliquus under light-dark cycles and nitrogen limitation
Carreres, B. M. (Creator) & Martens, D. E. (Creator), Wageningen University & Research, 30 May 2019
https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-7016/
Dataset
Scenedesmus Obliquus UTEX393 draft genome
Carreres, B. M. (Creator), de Jaeger, L. (Creator), Springer, J. (Creator), Barbosa, M. J. (Creator), Breuer, G. (Creator), van den End, E. J. (Creator), Kleinegris, D. M. M. (Creator), Schäffers, I. (Creator), Wolbert, E. J. H. (Creator), Zhang, H. (Creator), Lamers, P. P. (Creator), Martins dos Santos, V. A. P. (Creator), Wijffels, R. H. (Creator), Eggink, G. (Creator), Schaap, P. J. (Creator) & Martens, D. E. (Creator), Wageningen University, 1 Nov 2016
https://www.ebi.ac.uk/ena/data/view/PRJEB15865
Dataset

Systems Biology of microalgae as photosynthetic platform for tailored production of chemical building blocks and biomass (KB-17-003.02-029)
Martins dos Santos, V. (Project Leader)
1/01/13 → 31/12/13
Project: LVVN project

Cite this

@article{cc76bb11692e4547b9edf1a6ceb25bad,

title = "The diurnal transcriptional landscape of the microalga Tetradesmus obliquus",

abstract = "Tetradesmus obliquus is a promising oleaginous microalga. We functionally annotated its genome and characterized the transcriptional landscape of T. obliquus adapted to 16:8 h light dark (LD) cycles in turbidostat culture conditions at very high temporal resolution (1 h intervals). Revealing a cycle of cellular events, six distinct expression profiles were obtained, each with transcriptional phenotypes correlating with measurements of biochemical composition. The impact of starch deficiency was studied using the starchless mutant slm1. Significant changes in the transcriptional landscape were observed. Starch deficiency resulted in incapacity to supply energy during the dark period, resulting in a shift of energy demanding processes to an earlier or later time point. Our study provides new perspectives on the role of starch and the adaptation to LD cycles of oleaginous microalgae.",

keywords = "Day/night cycles, Diurnal transcription changes, Microalgae, Photosynthetic efficiency, Scenedesmus obliquus, starchless mutant",

author = "Carreres, {Benoit M.} and {Mitsue Le{\'o}n-Saiki}, G. and Schaap, {Peter J.} and Remmers, {Ilse M.} and {van der Veen}, Douwe and {Martins dos Santos}, {Vitor A.P.} and Wijffels, {Ren{\'e} H.} and Martens, {Dirk E.} and Maria Suarez-Diez",

year = "2019",

month = jun,

day = "1",

doi = "10.1101/425934",

language = "English",

volume = "40",

journal = "Algal Research",

issn = "2211-9264",

publisher = "Elsevier",

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TY - JOUR

T1 - The diurnal transcriptional landscape of the microalga Tetradesmus obliquus

AU - Carreres, Benoit M.

AU - Mitsue León-Saiki, G.

AU - Schaap, Peter J.

AU - Remmers, Ilse M.

AU - van der Veen, Douwe

AU - Martins dos Santos, Vitor A.P.

AU - Wijffels, René H.

AU - Martens, Dirk E.

AU - Suarez-Diez, Maria

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Tetradesmus obliquus is a promising oleaginous microalga. We functionally annotated its genome and characterized the transcriptional landscape of T. obliquus adapted to 16:8 h light dark (LD) cycles in turbidostat culture conditions at very high temporal resolution (1 h intervals). Revealing a cycle of cellular events, six distinct expression profiles were obtained, each with transcriptional phenotypes correlating with measurements of biochemical composition. The impact of starch deficiency was studied using the starchless mutant slm1. Significant changes in the transcriptional landscape were observed. Starch deficiency resulted in incapacity to supply energy during the dark period, resulting in a shift of energy demanding processes to an earlier or later time point. Our study provides new perspectives on the role of starch and the adaptation to LD cycles of oleaginous microalgae.

AB - Tetradesmus obliquus is a promising oleaginous microalga. We functionally annotated its genome and characterized the transcriptional landscape of T. obliquus adapted to 16:8 h light dark (LD) cycles in turbidostat culture conditions at very high temporal resolution (1 h intervals). Revealing a cycle of cellular events, six distinct expression profiles were obtained, each with transcriptional phenotypes correlating with measurements of biochemical composition. The impact of starch deficiency was studied using the starchless mutant slm1. Significant changes in the transcriptional landscape were observed. Starch deficiency resulted in incapacity to supply energy during the dark period, resulting in a shift of energy demanding processes to an earlier or later time point. Our study provides new perspectives on the role of starch and the adaptation to LD cycles of oleaginous microalgae.

KW - Day/night cycles

KW - Diurnal transcription changes

KW - Microalgae

KW - Photosynthetic efficiency

KW - Scenedesmus obliquus

KW - starchless mutant

U2 - 10.1101/425934

DO - 10.1101/425934

M3 - Article

AN - SCOPUS:85064223596

SN - 2211-9264

VL - 40

JO - Algal Research

JF - Algal Research

M1 - 101477

ER -

The diurnal transcriptional landscape of the microalga Tetradesmus obliquus

Abstract

Keywords

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Datasets

Transcriptional and physiological diurnal responses of Tetradesmus obliquus under light-dark cycles and nitrogen limitation

Scenedesmus Obliquus UTEX393 draft genome

Projects

Systems Biology of microalgae as photosynthetic platform for tailored production of chemical building blocks and biomass (KB-17-003.02-029)

Cite this