The Distribution of Mobile Genetic Elements (MGEs) in MRSA CC398 Is Associated with Both Host and Country

A.J. McCarthy; A.A. Witney; K.A. Gould; A. Moodley; L. Guardabassi; A. Voss; O. Denis; E.M. Broens; J. Hinds; J.A. Lindsay

doi:10.1093/gbe/evr092

The Distribution of Mobile Genetic Elements (MGEs) in MRSA CC398 Is Associated with Both Host and Country

A.J. McCarthy, A.A. Witney, K.A. Gould, A. Moodley, L. Guardabassi, A. Voss, O. Denis, E.M. Broens, J. Hinds, J.A. Lindsay

Research output: Contribution to journal › Article › Academic › peer-review

79 Citations (Scopus)

Abstract

Methicillin-resistant Staphylococcus aureus clonal complex (CC) 398 has emerged from pigs to cause human infections in Europe and North America. We used a new 62-strain S. aureus microarray (SAM-62) to compare genomes of isolates from three geographical areas ( Belgium, Denmark, and Netherlands) to understand how CC398 colonizes different mammalian hosts. The core genomes of 44 pig isolates and 32 isolates from humans did not vary. However, mobile genetic element (MGE) distribution was variable including SCCmec. phi 3 bacteriophage and human specificity genes (chp, sak, scn) were found in invasive human but not pig isolates. SaP15 and putative ruminant specificity gene variants (vwb and scn) were common but not pig specific. Virulence and resistance gene carriage was host associated but country specific. We conclude MGE exchange is frequent in CC398 and greatest among populations in close contact. This feature may help determine epidemiological associations among isolates of the same lineage.

Original language	English
Pages (from-to)	1164-1174
Journal	Genome Biology and Evolution
Volume	3
DOIs	https://doi.org/10.1093/gbe/evr092
Publication status	Published - 2011

Keywords

resistant staphylococcus-aureus
factor-binding-protein
methicillin-resistant
comparative genomics
st398
pigs
infection
humans
specificity
adaptation

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title = "The Distribution of Mobile Genetic Elements (MGEs) in MRSA CC398 Is Associated with Both Host and Country",

abstract = "Methicillin-resistant Staphylococcus aureus clonal complex (CC) 398 has emerged from pigs to cause human infections in Europe and North America. We used a new 62-strain S. aureus microarray (SAM-62) to compare genomes of isolates from three geographical areas ( Belgium, Denmark, and Netherlands) to understand how CC398 colonizes different mammalian hosts. The core genomes of 44 pig isolates and 32 isolates from humans did not vary. However, mobile genetic element (MGE) distribution was variable including SCCmec. phi 3 bacteriophage and human specificity genes (chp, sak, scn) were found in invasive human but not pig isolates. SaP15 and putative ruminant specificity gene variants (vwb and scn) were common but not pig specific. Virulence and resistance gene carriage was host associated but country specific. We conclude MGE exchange is frequent in CC398 and greatest among populations in close contact. This feature may help determine epidemiological associations among isolates of the same lineage.",

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author = "A.J. McCarthy and A.A. Witney and K.A. Gould and A. Moodley and L. Guardabassi and A. Voss and O. Denis and E.M. Broens and J. Hinds and J.A. Lindsay",

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AU - McCarthy, A.J.

AU - Witney, A.A.

AU - Gould, K.A.

AU - Moodley, A.

AU - Guardabassi, L.

AU - Voss, A.

AU - Denis, O.

AU - Broens, E.M.

AU - Hinds, J.

AU - Lindsay, J.A.

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N2 - Methicillin-resistant Staphylococcus aureus clonal complex (CC) 398 has emerged from pigs to cause human infections in Europe and North America. We used a new 62-strain S. aureus microarray (SAM-62) to compare genomes of isolates from three geographical areas ( Belgium, Denmark, and Netherlands) to understand how CC398 colonizes different mammalian hosts. The core genomes of 44 pig isolates and 32 isolates from humans did not vary. However, mobile genetic element (MGE) distribution was variable including SCCmec. phi 3 bacteriophage and human specificity genes (chp, sak, scn) were found in invasive human but not pig isolates. SaP15 and putative ruminant specificity gene variants (vwb and scn) were common but not pig specific. Virulence and resistance gene carriage was host associated but country specific. We conclude MGE exchange is frequent in CC398 and greatest among populations in close contact. This feature may help determine epidemiological associations among isolates of the same lineage.

AB - Methicillin-resistant Staphylococcus aureus clonal complex (CC) 398 has emerged from pigs to cause human infections in Europe and North America. We used a new 62-strain S. aureus microarray (SAM-62) to compare genomes of isolates from three geographical areas ( Belgium, Denmark, and Netherlands) to understand how CC398 colonizes different mammalian hosts. The core genomes of 44 pig isolates and 32 isolates from humans did not vary. However, mobile genetic element (MGE) distribution was variable including SCCmec. phi 3 bacteriophage and human specificity genes (chp, sak, scn) were found in invasive human but not pig isolates. SaP15 and putative ruminant specificity gene variants (vwb and scn) were common but not pig specific. Virulence and resistance gene carriage was host associated but country specific. We conclude MGE exchange is frequent in CC398 and greatest among populations in close contact. This feature may help determine epidemiological associations among isolates of the same lineage.

KW - resistant staphylococcus-aureus

KW - factor-binding-protein

KW - methicillin-resistant

KW - comparative genomics

KW - st398

KW - pigs

KW - infection

KW - humans

KW - specificity

KW - adaptation

U2 - 10.1093/gbe/evr092

DO - 10.1093/gbe/evr092

M3 - Article

SN - 1759-6653

VL - 3

SP - 1164

EP - 1174

JO - Genome Biology and Evolution

JF - Genome Biology and Evolution

ER -

The Distribution of Mobile Genetic Elements (MGEs) in MRSA CC398 Is Associated with Both Host and Country

Abstract

Keywords

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