The development of an efficient cultivar-independent plant regeneration system from callus derived from both apical and non-apical root segments of garlic (Allium sativum L.)

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Abstract

Callus induction and later plant regeneration were studied in four widely grown garlic (Allium sativum L.) cultivars from Europe. Root segments from in vitro plantlets were used as starting material. In addition to cultivar effects, the effects of auxin and cytokinin levels and the position of the segments on the root were studied. There were no statistically significant differences among cultivars for the number of root segments that induced callus in the two series of experiments. The average induction frequency was 34.7% in the first series of experiments. Callus induction on apical root segments was significantly higher compared to callus induction on non-apical root segments in the second series of experiments. Two months after callus induction, callus lines were transferred to a regeneration medium consisting of Murashige and Skoog basal medium supplemented with 30 g l-1 sucrose and 1 mg l-1 (4.6 M) kinetin. Calluses derived from different experiments were quite uniform with respect to their regeneration potential. Also it was found that our regeneration system was cultivar-independent. The average shoot regeneration frequency was 17.9% in the first series of experiments. Highly significant differences were found in the frequency of shoot regeneration among different callus induction treatments. When the cytokinin 6-(,-dimethylallylamino)purine (0.1 mg l-1: 0.5 M) was present during callus induction, shoot regeneration ranged from 30.10 to 47.60%. Shoot regeneration from callus induced on non-apical segments was higher, although not significant, compared to callus induction from apical root segments in the second series of experiments. All in all, an efficient callus induction and plant regeneration system was developed from both apical and non-apical segments taken along the entire length of the roots. This system has potential to be used for garlic transformation.
Original languageEnglish
Pages (from-to)288-292
JournalIn Vitro Cellular and Developmental Biology. Plant
Volume39
Issue number3
DOIs
Publication statusPublished - 2003

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Garlic
Allium sativum
Bony Callus
garlic
Regeneration
callus
cultivars
Cytokinins
shoots
cytokinins
Kinetin
Indoleacetic Acids
purines
kinetin
Sucrose
plantlets
auxins

Keywords

  • somatic embryogenesis
  • shoot regeneration
  • tissue-culture
  • cepa
  • tip
  • transformation
  • primordia

Cite this

@article{e32dd29f92ad44208070bd8195c97e53,
title = "The development of an efficient cultivar-independent plant regeneration system from callus derived from both apical and non-apical root segments of garlic (Allium sativum L.)",
abstract = "Callus induction and later plant regeneration were studied in four widely grown garlic (Allium sativum L.) cultivars from Europe. Root segments from in vitro plantlets were used as starting material. In addition to cultivar effects, the effects of auxin and cytokinin levels and the position of the segments on the root were studied. There were no statistically significant differences among cultivars for the number of root segments that induced callus in the two series of experiments. The average induction frequency was 34.7{\%} in the first series of experiments. Callus induction on apical root segments was significantly higher compared to callus induction on non-apical root segments in the second series of experiments. Two months after callus induction, callus lines were transferred to a regeneration medium consisting of Murashige and Skoog basal medium supplemented with 30 g l-1 sucrose and 1 mg l-1 (4.6 M) kinetin. Calluses derived from different experiments were quite uniform with respect to their regeneration potential. Also it was found that our regeneration system was cultivar-independent. The average shoot regeneration frequency was 17.9{\%} in the first series of experiments. Highly significant differences were found in the frequency of shoot regeneration among different callus induction treatments. When the cytokinin 6-(,-dimethylallylamino)purine (0.1 mg l-1: 0.5 M) was present during callus induction, shoot regeneration ranged from 30.10 to 47.60{\%}. Shoot regeneration from callus induced on non-apical segments was higher, although not significant, compared to callus induction from apical root segments in the second series of experiments. All in all, an efficient callus induction and plant regeneration system was developed from both apical and non-apical segments taken along the entire length of the roots. This system has potential to be used for garlic transformation.",
keywords = "somatic embryogenesis, shoot regeneration, tissue-culture, cepa, tip, transformation, primordia",
author = "S.J. Zheng and G. Henken and F.A. Krens and C. Kik",
year = "2003",
doi = "10.1079/IVP2002378",
language = "English",
volume = "39",
pages = "288--292",
journal = "In Vitro Cellular and Developmental Biology. Plant",
issn = "1054-5476",
publisher = "Society for in vitro biology",
number = "3",

}

TY - JOUR

T1 - The development of an efficient cultivar-independent plant regeneration system from callus derived from both apical and non-apical root segments of garlic (Allium sativum L.)

AU - Zheng, S.J.

AU - Henken, G.

AU - Krens, F.A.

AU - Kik, C.

PY - 2003

Y1 - 2003

N2 - Callus induction and later plant regeneration were studied in four widely grown garlic (Allium sativum L.) cultivars from Europe. Root segments from in vitro plantlets were used as starting material. In addition to cultivar effects, the effects of auxin and cytokinin levels and the position of the segments on the root were studied. There were no statistically significant differences among cultivars for the number of root segments that induced callus in the two series of experiments. The average induction frequency was 34.7% in the first series of experiments. Callus induction on apical root segments was significantly higher compared to callus induction on non-apical root segments in the second series of experiments. Two months after callus induction, callus lines were transferred to a regeneration medium consisting of Murashige and Skoog basal medium supplemented with 30 g l-1 sucrose and 1 mg l-1 (4.6 M) kinetin. Calluses derived from different experiments were quite uniform with respect to their regeneration potential. Also it was found that our regeneration system was cultivar-independent. The average shoot regeneration frequency was 17.9% in the first series of experiments. Highly significant differences were found in the frequency of shoot regeneration among different callus induction treatments. When the cytokinin 6-(,-dimethylallylamino)purine (0.1 mg l-1: 0.5 M) was present during callus induction, shoot regeneration ranged from 30.10 to 47.60%. Shoot regeneration from callus induced on non-apical segments was higher, although not significant, compared to callus induction from apical root segments in the second series of experiments. All in all, an efficient callus induction and plant regeneration system was developed from both apical and non-apical segments taken along the entire length of the roots. This system has potential to be used for garlic transformation.

AB - Callus induction and later plant regeneration were studied in four widely grown garlic (Allium sativum L.) cultivars from Europe. Root segments from in vitro plantlets were used as starting material. In addition to cultivar effects, the effects of auxin and cytokinin levels and the position of the segments on the root were studied. There were no statistically significant differences among cultivars for the number of root segments that induced callus in the two series of experiments. The average induction frequency was 34.7% in the first series of experiments. Callus induction on apical root segments was significantly higher compared to callus induction on non-apical root segments in the second series of experiments. Two months after callus induction, callus lines were transferred to a regeneration medium consisting of Murashige and Skoog basal medium supplemented with 30 g l-1 sucrose and 1 mg l-1 (4.6 M) kinetin. Calluses derived from different experiments were quite uniform with respect to their regeneration potential. Also it was found that our regeneration system was cultivar-independent. The average shoot regeneration frequency was 17.9% in the first series of experiments. Highly significant differences were found in the frequency of shoot regeneration among different callus induction treatments. When the cytokinin 6-(,-dimethylallylamino)purine (0.1 mg l-1: 0.5 M) was present during callus induction, shoot regeneration ranged from 30.10 to 47.60%. Shoot regeneration from callus induced on non-apical segments was higher, although not significant, compared to callus induction from apical root segments in the second series of experiments. All in all, an efficient callus induction and plant regeneration system was developed from both apical and non-apical segments taken along the entire length of the roots. This system has potential to be used for garlic transformation.

KW - somatic embryogenesis

KW - shoot regeneration

KW - tissue-culture

KW - cepa

KW - tip

KW - transformation

KW - primordia

U2 - 10.1079/IVP2002378

DO - 10.1079/IVP2002378

M3 - Article

VL - 39

SP - 288

EP - 292

JO - In Vitro Cellular and Developmental Biology. Plant

JF - In Vitro Cellular and Developmental Biology. Plant

SN - 1054-5476

IS - 3

ER -