The development of a novel diagnostic PCR for Madurella mycetomatis using a comparative genome approach

Wilson Lim, Emmanuel Siddig, Kimberly Eadie, Bertrand Nyuykonge, Sarah Ahmed, Ahmed Fahal, Annelies Verbon, Sandra Smit, Wendy W.J. van de Sande*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

BACKGROUND: Eumycetoma is a neglected tropical disease most commonly caused by the fungus Madurella mycetomatis. Identification of eumycetoma causative agents can only be reliably performed by molecular identification, most commonly by species-specific PCR. The current M. mycetomatis specific PCR primers were recently discovered to cross-react with Madurella pseudomycetomatis. Here, we used a comparative genome approach to develop a new M. mycetomatis specific PCR for species identification. METHODOLOGY: Predicted-protein coding sequences unique to M. mycetomatis were first identified in BLASTCLUST based on E-value, size and presence of orthologues. Primers were then developed for 16 unique sequences and evaluated against 60 M. mycetomatis isolates and other eumycetoma causing agents including the Madurella sibling species. Out of the 16, only one was found to be specific to M. mycetomatis. CONCLUSION: We have discovered a predicted-protein coding sequence unique to M. mycetomatis and have developed a new species-specific PCR to be used as a novel diagnostic marker for M. mycetomatis.

Original languageEnglish
Article numbere0008897
JournalPLoS Neglected Tropical Diseases
Volume14
Issue number12
DOIs
Publication statusPublished - 1 Dec 2020

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