The degradation of HFR1, a putative bHLH class transcription factor involved in light signaling, is regulated by phosphorylation and requires COP1

P.D. Duek, M.V. Elmer, V.R. van Oosten, C. Fankhauser

Research output: Contribution to journalArticleAcademicpeer-review

149 Citations (Scopus)

Abstract

All developmental transitions throughout the life cycle of a plant are influenced by light. In Arabidopsis, multiple photoreceptors including the UV-A/blue-sensing cryptochromes (cry1-2) and the red/far-red responsive phytochromes (phyA-E) monitor the ambient light conditions [1 and 2]. Light-regulated protein stability is a major control point of photomorphogenesis [3]. The ubiquitin E3 ligase COP1 (nstitutively hotomorphogenic 1) regulates the stability of several light-signaling components [4, 5 and 6]. HFR1 (long ypocotyl in ar-ed light) is a putative transcription factor with a bHLH domain acting downstream of both phyA and the cryptochromes [7, 8 and 9]. HFR1 is closely related to PIF1, PIF3, and PIF4 (hytochrome nteracting actor 1, 3 and 4), but in contrast to the latter three, there is no evidence for a direct interaction between HFR1 and the phytochromes [7, 10, 11 and 12]. Here, we show that the protein abundance of HFR1 is tightly controlled by light. HFR1 is an unstable phosphoprotein, particularly in the dark. The proteasome and COP1 are required in vivo to degrade phosphorylated HFR1. In addition, HFR1 can interact with COP1, consistent with the idea of COP1 directly mediating HFR1 degradation. We identify a domain, conserved among several bHLH class proteins involved in light signaling [13 and 14], as a determinant of HFR1 stability. Our physiological experiments indicate that the control of HFR1 protein abundance is important for a normal de-etiolation response
Original languageEnglish
Pages (from-to)2296-2301
JournalCurrent Biology
Volume14
Issue number24
DOIs
Publication statusPublished - 2004

Keywords

  • loop-helix protein
  • phytochrome-a
  • arabidopsis cryptochromes
  • branch pathway
  • factor family
  • hy5
  • photomorphogenesis
  • ubiquitination
  • thaliana
  • binding

Fingerprint Dive into the research topics of 'The degradation of HFR1, a putative bHLH class transcription factor involved in light signaling, is regulated by phosphorylation and requires COP1'. Together they form a unique fingerprint.

  • Cite this