The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways

Diana Z. Sousa, Michael Visser, Antonie H. Van Gelder, Sjef Boeren, Mervin M. Pieterse, Martijn W.H. Pinkse, Peter D.E.M. Verhaert, Carsten Vogt, Steffi Franke, Steffen Kümmel, Alfons J.M. Stams

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4 Citations (Scopus)

Abstract

Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.
LanguageEnglish
Article number239
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - 16 Jan 2018

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Desulfotomaculum
Sulfates
Methanol
sulfates
methyl alcohol
Cobalt
Methyltransferases
dehydrogenases
cobalt
aerobes
Methanogens
Vitamin B 12
Fractionation
vitamins
Metabolism
Isotopes
Microorganisms
Proteomics
Formaldehyde
metabolism

Cite this

Sousa, Diana Z. ; Visser, Michael ; Van Gelder, Antonie H. ; Boeren, Sjef ; Pieterse, Mervin M. ; Pinkse, Martijn W.H. ; Verhaert, Peter D.E.M. ; Vogt, Carsten ; Franke, Steffi ; Kümmel, Steffen ; Stams, Alfons J.M. / The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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abstract = "Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.",
author = "Sousa, {Diana Z.} and Michael Visser and {Van Gelder}, {Antonie H.} and Sjef Boeren and Pieterse, {Mervin M.} and Pinkse, {Martijn W.H.} and Verhaert, {Peter D.E.M.} and Carsten Vogt and Steffi Franke and Steffen K{\"u}mmel and Stams, {Alfons J.M.}",
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The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways. / Sousa, Diana Z.; Visser, Michael; Van Gelder, Antonie H.; Boeren, Sjef; Pieterse, Mervin M.; Pinkse, Martijn W.H.; Verhaert, Peter D.E.M.; Vogt, Carsten; Franke, Steffi; Kümmel, Steffen; Stams, Alfons J.M.

In: Nature Communications, Vol. 9, No. 1, 239, 16.01.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways

AU - Sousa, Diana Z.

AU - Visser, Michael

AU - Van Gelder, Antonie H.

AU - Boeren, Sjef

AU - Pieterse, Mervin M.

AU - Pinkse, Martijn W.H.

AU - Verhaert, Peter D.E.M.

AU - Vogt, Carsten

AU - Franke, Steffi

AU - Kümmel, Steffen

AU - Stams, Alfons J.M.

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N2 - Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.

AB - Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.

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