The cytoplasmic domain of tomato spotted witl virus Gn glycoprotein is required for Golgi localisation and interaction with Gc

M. Snippe, L. Smeenk, R.W. Goldbach, R.J.M. Kormelink

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Envelopment of tomato spotted wilt virus nucleocapsids occurs at the Golgi stacks of infected cells. This is also the place where the two membrane glycoproteins Gn and Gc accumulate upon coexpression. The required Golgi retention signal has previously been demonstrated to reside within Gn. Using a series of truncated Gn proteins, the Golgi retention signal was mapped to a stretch of 10 amino acids on this protein's cytoplasmic tail, 20 residues downstream the transmembrane domain. Studies on the intracellular distribution of chimeric Gc proteins in which the cytoplasmic tail and/or transmembrane domain were exchanged by those from Gn, demonstrated the additional requirement of the Gn transmembrane domain for Golgi targeting. Truncated Gn constructs lacking the C-terminal 20 amino acids but still localising to the Golgi were no longer able to redirect Gc, suggesting the requirement of this domain for interaction with Gc.
Original languageEnglish
Pages (from-to)272-279
Number of pages8
JournalVirology
Volume363
Issue number2
DOIs
Publication statusPublished - 2007

Keywords

  • intracellular-transport
  • hantaan virus
  • retention
  • g1
  • signal
  • coexpression
  • trafficking
  • expression
  • complex
  • cells

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