The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies

Fengqiao Zhou; Wenhua Kuang; Xi Wang; Dianhai Hou; Huachao Huang; Xiulian Sun; Fei Deng; Hualin Wang; Monqiue M. van Oers; Manli Wang; Zhihong Hu

doi:10.1016/j.virol.2019.06.016

The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies

Fengqiao Zhou, Wenhua Kuang, Xi Wang, Dianhai Hou, Huachao Huang, Xiulian Sun, Fei Deng, Hualin Wang, Monqiue M. van Oers, Manli Wang, Zhihong Hu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The baculovirus core gene vp91 has been reported to be essential for nucleocapsid assembly and oral infection. Here, we studied the function of vp91 by analyzing its homologue, ha76, in Helicoverpa armigera nucleopolyhedrovirus (HearNPV). HA76 was expressed at the late stage of HearNPV infection; deletion of ha76 showed that the gene is required for budded virus production. A series of recombinants with truncated ha76 was constructed and analyzed in vitro and in vivo. The results showed that the region encoding the C-terminus of HA76 was essential for nucleocapsid assembly, whereas the N-terminal cysteine-rich region was responsible for oral infection. Electron microscope analyses further showed that the cysteine-rich region contributed to morphogenesis of occlusion bodies (OBs), with amino acids 136–223 of HA76 being critical for this function. The results revealed a novel function of VP91 and suggested that the impact on OB morphogenesis is partially related to oral infectivity.

Original language	English
Pages (from-to)	144-153
Journal	Virology
Volume	535
DOIs	https://doi.org/10.1016/j.virol.2019.06.016
Publication status	Published - Sep 2019

Keywords

Cysteine-rich region
Disulfide bond
HA76
Occlusion body (OB)
Oral infection
P33
Per os infectivity factor (PIF)
PIF8
Polyhedra
VP91

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@article{fa8929c6f48e4e1fae5d856052d44633,

title = "The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies",

abstract = "The baculovirus core gene vp91 has been reported to be essential for nucleocapsid assembly and oral infection. Here, we studied the function of vp91 by analyzing its homologue, ha76, in Helicoverpa armigera nucleopolyhedrovirus (HearNPV). HA76 was expressed at the late stage of HearNPV infection; deletion of ha76 showed that the gene is required for budded virus production. A series of recombinants with truncated ha76 was constructed and analyzed in vitro and in vivo. The results showed that the region encoding the C-terminus of HA76 was essential for nucleocapsid assembly, whereas the N-terminal cysteine-rich region was responsible for oral infection. Electron microscope analyses further showed that the cysteine-rich region contributed to morphogenesis of occlusion bodies (OBs), with amino acids 136–223 of HA76 being critical for this function. The results revealed a novel function of VP91 and suggested that the impact on OB morphogenesis is partially related to oral infectivity.",

keywords = "Cysteine-rich region, Disulfide bond, HA76, Occlusion body (OB), Oral infection, P33, Per os infectivity factor (PIF), PIF8, Polyhedra, VP91",

author = "Fengqiao Zhou and Wenhua Kuang and Xi Wang and Dianhai Hou and Huachao Huang and Xiulian Sun and Fei Deng and Hualin Wang and {van Oers}, {Monqiue M.} and Manli Wang and Zhihong Hu",

year = "2019",

month = sep,

doi = "10.1016/j.virol.2019.06.016",

language = "English",

volume = "535",

pages = "144--153",

journal = "Virology",

issn = "0042-6822",

publisher = "Elsevier",

}

The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies. / Zhou, Fengqiao; Kuang, Wenhua; Wang, Xi; Hou, Dianhai; Huang, Huachao; Sun, Xiulian; Deng, Fei; Wang, Hualin; van Oers, Monqiue M.; Wang, Manli; Hu, Zhihong.

In: Virology, Vol. 535, 09.2019, p. 144-153.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies

AU - Zhou, Fengqiao

AU - Kuang, Wenhua

AU - Wang, Xi

AU - Hou, Dianhai

AU - Huang, Huachao

AU - Sun, Xiulian

AU - Deng, Fei

AU - Wang, Hualin

AU - van Oers, Monqiue M.

AU - Wang, Manli

AU - Hu, Zhihong

PY - 2019/9

Y1 - 2019/9

N2 - The baculovirus core gene vp91 has been reported to be essential for nucleocapsid assembly and oral infection. Here, we studied the function of vp91 by analyzing its homologue, ha76, in Helicoverpa armigera nucleopolyhedrovirus (HearNPV). HA76 was expressed at the late stage of HearNPV infection; deletion of ha76 showed that the gene is required for budded virus production. A series of recombinants with truncated ha76 was constructed and analyzed in vitro and in vivo. The results showed that the region encoding the C-terminus of HA76 was essential for nucleocapsid assembly, whereas the N-terminal cysteine-rich region was responsible for oral infection. Electron microscope analyses further showed that the cysteine-rich region contributed to morphogenesis of occlusion bodies (OBs), with amino acids 136–223 of HA76 being critical for this function. The results revealed a novel function of VP91 and suggested that the impact on OB morphogenesis is partially related to oral infectivity.

AB - The baculovirus core gene vp91 has been reported to be essential for nucleocapsid assembly and oral infection. Here, we studied the function of vp91 by analyzing its homologue, ha76, in Helicoverpa armigera nucleopolyhedrovirus (HearNPV). HA76 was expressed at the late stage of HearNPV infection; deletion of ha76 showed that the gene is required for budded virus production. A series of recombinants with truncated ha76 was constructed and analyzed in vitro and in vivo. The results showed that the region encoding the C-terminus of HA76 was essential for nucleocapsid assembly, whereas the N-terminal cysteine-rich region was responsible for oral infection. Electron microscope analyses further showed that the cysteine-rich region contributed to morphogenesis of occlusion bodies (OBs), with amino acids 136–223 of HA76 being critical for this function. The results revealed a novel function of VP91 and suggested that the impact on OB morphogenesis is partially related to oral infectivity.

KW - Cysteine-rich region

KW - Disulfide bond

KW - HA76

KW - Occlusion body (OB)

KW - Oral infection

KW - P33

KW - Per os infectivity factor (PIF)

KW - PIF8

KW - Polyhedra

KW - VP91

U2 - 10.1016/j.virol.2019.06.016

DO - 10.1016/j.virol.2019.06.016

M3 - Article

AN - SCOPUS:85068549578

VL - 535

SP - 144

EP - 153

JO - Virology

JF - Virology

SN - 0042-6822

ER -