The Cyst Nematode SPRYSEC Protein RBP-1 Elicits Gpa2- and RanGAP2-Dependent Plant Cell Death

M.A. Sacco, K.B. Koropacka, E. Grenier, M.J. Jaubert, A. Blanchard, A. Goverse, G. Smant, P. Moffett

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104 Citations (Scopus)

Abstract

Plant NB-LRR proteins confer robust protection against microbes and metazoan parasites by recognizing pathogen-derived avirulence (Avr) proteins that are delivered to the host cytoplasm. Microbial Avr proteins usually function as virulence factors in compatible interactions; however, little is known about the types of metazoan proteins recognized by NB-LRR proteins and their relationship with virulence. In this report, we demonstrate that the secreted protein RBP-1 from the potato cyst nematode Globodera pallida elicits defense responses, including cell death typical of a hypersensitive response (HR), through the NB-LRR protein Gpa2. Gp-Rbp-1 variants from G. pallida populations both virulent and avirulent to Gpa2 demonstrated a high degree of polymorphism, with positive selection detected at numerous sites. All Gp-RBP-1 protein variants from an avirulent population were recognized by Gpa2, whereas virulent populations possessed Gp-RBP-1 protein variants both recognized and non-recognized by Gpa2. Recognition of Gp-RBP-1 by Gpa2 correlated to a single amino acid polymorphism at position 187 in the Gp-RBP-1 SPRY domain. Gp-RBP-1 expressed from Potato virus X elicited Gpa2-mediated defenses that required Ran GTPase-activating protein 2 (RanGAP2), a protein known to interact with the Gpa2 N terminus. Tethering RanGAP2 and Gp-RBP-1 variants via fusion proteins resulted in an enhancement of Gpa2-mediated responses. However, activation of Gpa2 was still dependent on the recognition specificity conferred by amino acid 187 and the Gpa2 LRR domain. These results suggest a two-tiered process wherein RanGAP2 mediates an initial interaction with pathogen-delivered Gp-RBP-1 proteins but where the Gpa2 LRR determines which of these interactions will be productive
Original languageEnglish
Article numbere1000564
JournalPLoS Pathogens
Volume5
Issue number8
DOIs
Publication statusPublished - 2009

Keywords

  • disease resistance genes
  • rich repeat protein
  • nb-lrr protein
  • globodera-pallida
  • downy mildew
  • sequence alignment
  • avirulence genes
  • virus-resistance
  • potato
  • arabidopsis

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    Sacco, M. A., Koropacka, K. B., Grenier, E., Jaubert, M. J., Blanchard, A., Goverse, A., Smant, G., & Moffett, P. (2009). The Cyst Nematode SPRYSEC Protein RBP-1 Elicits Gpa2- and RanGAP2-Dependent Plant Cell Death. PLoS Pathogens, 5(8), [e1000564]. https://doi.org/10.1371/journal.ppat.1000564