The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts

M.C. Hesselman, J.J. Koehorst, T. Slijkhuis, F. Hugenholtz, D.I. Odoni, M.W.J. van Passel

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biological Parts harbours such a repository of standardized parts, and thereby facilitates the combination of complex molecular modules to novel genetic circuits and devices. However, since finding the best parts for a pre-determined genetic design can be time consuming, we devised the Constructor, a web tool that recommends the smallest number of cloning steps for pre-designed circuits, and implements user-defined quality checks.We present the Constructor (www.systemsbiology.nl/the_constructor) as a constructive web tool that simplifies the in silico assembly of pre-designed gene circuitries from standard parts, reducing both planning and subsequent cloning time
Original languageEnglish
Article number14
Number of pages6
JournalJournal of Biological Engineering
Volume6
DOIs
Publication statusPublished - 2012

Fingerprint Dive into the research topics of 'The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts'. Together they form a unique fingerprint.

Cite this