The construction of a linkage map of Alstroemeria aurea by AFLP markers

T.H. Han, H.J. van Eck, M.J. de Jeu, E. Jacobsen

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7 Citations (Scopus)


An AFLP based linkage map has been generated for the ornamental cropspecies Alstroemeria aurea. In view of the large genome size of Alstroemeria (25,000 Mb) the number of selective nucleotides for AFLPamplification was increased to EcoRI+4/MseI+4 to generatefingerprints of moderate complexity. In addition, markers were generatedwith the enzyme combination Sse/Mse, where Sse8387I is an8-cutter, thereby reducing AFLP template complexity. Segregation of 374AFLP polymorphisms was recorded in the F1 of an intraspecific A. aurea cross (A002 × A003). The map consisted of 8 A002 and 10A003 linkage groups with 122 and 214 markers covering 306.3 and605.6 cM, respectively. The two maps were integrated by using the21% of the AFLP markers that were heterozygous in both parents, and31% of the markers remained unlinked. Pollen color was assigned tolinkage group A002-6. The enzyme combinations EcoRI+4/MseI+4 and Sse+2/MseI+3 generated 80 and 30 clear bandsper lane with 16 and 9 polymorphic markers, respectively. Twenty percentof the EcoRI+4/MseI+4 primer combinations resulted infingerprints that were disturbed by a few excessively thick bands (55 outof 288 primer combinations). We conclude that fingerprints and markersgenerated with the eight-cutter enzyme Sse8387I, in combination with+2/+3 selective nucleotides (Sse+2/MseI+3) are superior toEcoRI+4/MseI+4
Original languageEnglish
Pages (from-to)153-164
Issue number2
Publication statusPublished - 2002

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