The complete nucleotide sequence of chrysanthemum stem necrosis virus

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5' and 3' termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates
Original languageEnglish
Pages (from-to)605-608
JournalArchives of Virology
Volume160
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • 1st report
  • tospovirus
  • identification
  • diversity
  • zucchini

Fingerprint Dive into the research topics of 'The complete nucleotide sequence of chrysanthemum stem necrosis virus'. Together they form a unique fingerprint.

  • Projects

    Cite this