The complete nucleotide sequence of chrysanthemum stem necrosis virus

A.M. Dullemans; J.Th.J. Verhoeven; R.J.M. Kormelink; R.A.A. van der Vlugt

doi:10.1007/s00705-014-2282-1

The complete nucleotide sequence of chrysanthemum stem necrosis virus

A.M. Dullemans^*, J.Th.J. Verhoeven, R.J.M. Kormelink, R.A.A. van der Vlugt

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

10 Citations (Scopus)

Abstract

The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5' and 3' termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates

Original language	English
Pages (from-to)	605-608
Journal	Archives of Virology
Volume	160
Issue number	2
DOIs	https://doi.org/10.1007/s00705-014-2282-1
Publication status	Published - 2015

Keywords

1st report
tospovirus
identification
diversity
zucchini

Access to Document

10.1007/s00705-014-2282-1

KV 1605-036 Fundament fytosanitair robuuste ketens (BO-50-002-053, BO-33.05-002-010)
Bonants, P. (Project Leader)
1/01/17 → 31/12/19
Project: LVVN project
QBOL: Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health
21/03/09 → 20/09/12
Project: EU research project

Cite this

@article{b116535756294514bcd20c3b52e1f8b1,

title = "The complete nucleotide sequence of chrysanthemum stem necrosis virus",

abstract = "The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5' and 3' termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates",

keywords = "1st report, tospovirus, identification, diversity, zucchini",

author = "A.M. Dullemans and J.Th.J. Verhoeven and R.J.M. Kormelink and {van der Vlugt}, R.A.A.",

year = "2015",

doi = "10.1007/s00705-014-2282-1",

language = "English",

volume = "160",

pages = "605--608",

journal = "Archives of Virology",

issn = "0304-8608",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - The complete nucleotide sequence of chrysanthemum stem necrosis virus

AU - Dullemans, A.M.

AU - Verhoeven, J.Th.J.

AU - Kormelink, R.J.M.

AU - van der Vlugt, R.A.A.

PY - 2015

Y1 - 2015

N2 - The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5' and 3' termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates

AB - The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5' and 3' termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates

KW - 1st report

KW - tospovirus

KW - identification

KW - diversity

KW - zucchini

U2 - 10.1007/s00705-014-2282-1

DO - 10.1007/s00705-014-2282-1

M3 - Article

SN - 0304-8608

VL - 160

SP - 605

EP - 608

JO - Archives of Virology

JF - Archives of Virology

IS - 2

ER -

The complete nucleotide sequence of chrysanthemum stem necrosis virus

Abstract

Keywords

Access to Document

Fingerprint

Projects

KV 1605-036 Fundament fytosanitair robuuste ketens (BO-50-002-053, BO-33.05-002-010)

QBOL: Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health

Cite this