The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses

R. Souza Richards, I.P. Adams, J.F. Kreuze, J. de Souza, W. Cuellar, A.M. Dullemans, R.A.A. van der Vlugt, R. Glover, U. Hany, M. Dickinson, N. Boonham

    Research output: Contribution to journalArticleAcademicpeer-review

    8 Citations (Scopus)

    Abstract

    The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence
    Original languageEnglish
    Pages (from-to)811-815
    JournalArchives of Virology
    Volume159
    Issue number4
    DOIs
    Publication statusPublished - 2014

    Keywords

    • complete nucleotide-sequence
    • grapevine-fanleaf-virus
    • arabis-mosaic-virus
    • n-terminal region
    • tobacco ringspot
    • cleavage site
    • in-vitro
    • polyprotein
    • organization
    • rna2

    Fingerprint

    Dive into the research topics of 'The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses'. Together they form a unique fingerprint.

    Cite this