The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses

R. Souza Richards; I.P. Adams; J.F. Kreuze; J. de Souza; W. Cuellar; A.M. Dullemans; R.A.A. van der Vlugt; R. Glover; U. Hany; M. Dickinson; N. Boonham

doi:10.1007/s00705-013-1871-8

The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses

R. Souza Richards, I.P. Adams, J.F. Kreuze, J. de Souza, W. Cuellar, A.M. Dullemans, R.A.A. van der Vlugt, R. Glover, U. Hany, M. Dickinson, N. Boonham

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Abstract

The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence

Original language	English
Pages (from-to)	811-815
Journal	Archives of Virology
Volume	159
Issue number	4
DOIs	https://doi.org/10.1007/s00705-013-1871-8
Publication status	Published - 2014

Keywords

complete nucleotide-sequence
grapevine-fanleaf-virus
arabis-mosaic-virus
n-terminal region
tobacco ringspot
cleavage site
in-vitro
polyprotein
organization
rna2

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Souza Richards, R., Adams, I. P., Kreuze, J. F., de Souza, J., Cuellar, W., Dullemans, A. M., van der Vlugt, R. A. A., Glover, R., Hany, U., Dickinson, M., & Boonham, N. (2014). The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses. Archives of Virology, 159(4), 811-815. https://doi.org/10.1007/s00705-013-1871-8

@article{a77d09e62155475b9f86e2788812515c,

title = "The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses",

abstract = "The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence",

keywords = "complete nucleotide-sequence, grapevine-fanleaf-virus, arabis-mosaic-virus, n-terminal region, tobacco ringspot, cleavage site, in-vitro, polyprotein, organization, rna2",

author = "{Souza Richards}, R. and I.P. Adams and J.F. Kreuze and {de Souza}, J. and W. Cuellar and A.M. Dullemans and {van der Vlugt}, R.A.A. and R. Glover and U. Hany and M. Dickinson and N. Boonham",

year = "2014",

doi = "10.1007/s00705-013-1871-8",

language = "English",

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Souza Richards, R, Adams, IP, Kreuze, JF, de Souza, J, Cuellar, W, Dullemans, AM , van der Vlugt, RAA, Glover, R, Hany, U, Dickinson, M & Boonham, N 2014, 'The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses', Archives of Virology, vol. 159, no. 4, pp. 811-815. https://doi.org/10.1007/s00705-013-1871-8

TY - JOUR

T1 - The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses

AU - Souza Richards, R.

AU - Adams, I.P.

AU - Kreuze, J.F.

AU - de Souza, J.

AU - Cuellar, W.

AU - Dullemans, A.M.

AU - van der Vlugt, R.A.A.

AU - Glover, R.

AU - Hany, U.

AU - Dickinson, M.

AU - Boonham, N.

PY - 2014

Y1 - 2014

N2 - The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence

AB - The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence

KW - complete nucleotide-sequence

KW - grapevine-fanleaf-virus

KW - arabis-mosaic-virus

KW - n-terminal region

KW - tobacco ringspot

KW - cleavage site

KW - in-vitro

KW - polyprotein

KW - organization

KW - rna2

U2 - 10.1007/s00705-013-1871-8

DO - 10.1007/s00705-013-1871-8

M3 - Article

VL - 159

SP - 811

EP - 815

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 4

ER -

The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses

Abstract

Keywords

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