The complement regulating protein SALSA in early life: diverse roles in amniotic fluid and intestine

M.P. Reichhardt, H. Jarva, M. de Been, J.M. Rodriguez, E.Q. Jimenez, V. Loimaranta, W.M. de Vos, S. Meri

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

fiThe salivary scavenger and agglutinin (SALSA), also known asgp340 and dmbt1, is produced by mucosal tissues throughoutthe body. SALSA is an anti-microbial and inflammation regulat-ing molecule located at the mucosal surfaces. It has been shownto regulate the classical and lectin pathways of the complementsystem through interactions with both C1q and MBL. It has alsobeen suggested to play a role in cell differentiation and epithelialpolarization. In the present study, a general screen of body secre-tions for SALSA revealed that SALSA was present in amniotic fluid(AF) and exceptionally abundant in both meconium and feces ofinfants. SALSA constituted up to 6–10% of the total protein amountin meconium, thus making it one of the most abundant proteins.Innate immunity is extremely important for both the mother in thecourse of maintaining a healthy pregnancy and for the infant in thevery early stages of life. The high levels of SALSA in AF, and espe-cially in the meconium and feces suggest a robust and importantfunction for SALSA both during the fetal development and in theinnate immune defense of infants. SALSA proteins in the AF andintestinal samples were polymorphic and exhibited varying pep-tide compositions. In particular, a different abundance of peptidescorresponding to functionally important structures were found inAF and intestinal SALSA. Peptides within the bacterial-binding partof the scavenger receptor cysteine-rich domains were more abun-dant in the intestinal SALSA. In contrast, SALSA present in the AF AFcontained relatively more peptides from the zona pellucida (ZP)domain, which is involved in cell differentiation. Our results havethus identified body compartments, where SALSA is particularlyabundant and suggest that SALSA exhibits varying functions in thedifferent locations.
Original languageEnglish
Title of host publicationAbstracts of the XXV International Complement Workshop, 14–18 September 2014, Rio de Janeiro, Brazil
PublisherElsevier
Pages252-252
Volume61
DOIs
Publication statusPublished - 2014
EventXXV International Complement Workshop, 14–18 September 2014, Rio de Janeiro, Brazil -
Duration: 14 Sep 201418 Sep 2014

Publication series

NameMolecular immunology
Number2
Volume61

Workshop

WorkshopXXV International Complement Workshop, 14–18 September 2014, Rio de Janeiro, Brazil
Period14/09/1418/09/14

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